Mammalian Oocyte Regulation

Methods and Protocols

  • Hayden A. Homer

Part of the Methods in Molecular Biology book series (MIMB, volume 957)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Xianfei Sun, Paula E. Cohen
    Pages 1-18
  3. Esther de Boer, Maria Jasin, Scott Keeney
    Pages 19-45
  4. Ji-hye Kim, Kei-ichiro Ishiguro, Nobuaki Kudo, Yoshinori Watanabe
    Pages 47-57
  5. Jing He, Andrew J. Childs, Jieqian Zhou, Richard A. Anderson
    Pages 59-75
  6. Julien Dumont, Marie-Hélène Verlhac
    Pages 107-120
  7. Paula Stein, Petr Svoboda, Richard M. Schultz
    Pages 135-151
  8. Jay M. Baltz, Hannah E. Corbett, Samantha Richard
    Pages 163-178
  9. Solon Riris, Suzanne Cawood, Liming Gui, Paul Serhal, Hayden A. Homer
    Pages 179-187
  10. Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, Tang K. Tang
    Pages 189-202
  11. Jean-Philippe Chambon, Khaled Hached, Katja Wassmann
    Pages 203-212
  12. Teresa Chiang, Michael A. Lampson
    Pages 249-253
  13. Zbigniew Polanski, Jacek Z. Kubiak
    Pages 255-265
  14. Eiji Mizutani, Atsuo Ogura, Teruhiko Wakayama
    Pages 285-300
  15. Esther de Boer, Maria Jasin, Scott Keeney
    Pages E1-E2
  16. Back Matter
    Pages 301-311

About this book


Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or egg) capable of fertilisation and embryonic development is of unrivalled complexity. The end-product of this remarkable developmental process is the “mature” egg, now replete with macromolecules and organelles, and poised to support embryonic development if fertilisation should occur. Given the importance of the oocyte for embryonic development, it is of no surprise that the oocyte holds centre stage in fertility clinics and greatly influences the outcome of assisted reproductive treatments (ARTs). Mammalian Oocyte Regulation: Methods and Protocols provides a highly diverse compendium of detailed methodologies - primarily focusing on the murine model but also including chapters on human oocytes - for enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Mammalian Oocyte Regulation: Methods and Protocols serves as an ideal guide to novice oocyte researchers, experts who wish to add another dimension to their established portfolio, as well as the wider research community.


embryonic development human oocyte mammalian eggs meiosis mouse oocyte

Editors and affiliations

  • Hayden A. Homer
    • 1
  1. 1.Dept. of Cell & Developmental BiologyUniversity College London Institute for Women's HealthLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-190-5
  • Online ISBN 978-1-62703-191-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site