Methods and Protocols

  • Carmen Buchrieser
  • Hubert Hilbi

Part of the Methods in Molecular Biology book series (MIMB, volume 954)

Table of contents

  1. Front Matter
    Pages i-xxviii
  2. Identification and Typing of Legionella in the Environment and During Disease

    1. Front Matter
      Pages 1-1
    2. Natalia A. Kozak, Claressa E. Lucas, Jonas M. Winchell
      Pages 3-25
    3. Sophie Jarraud, Ghislaine Descours, Christophe Ginevra, Gerard Lina, Jerome Etienne
      Pages 27-56
    4. Rodney M. Ratcliff
      Pages 57-72
    5. Carol Joseph
      Pages 73-86
    6. Christian Lück, Norman K. Fry, Jürgen H. Helbig, Sophie Jarraud, Timothy G. Harrison
      Pages 119-148
  3. Physiology and Genetics of Legionella

    1. Front Matter
      Pages 149-149
    2. Christa H. Chatfield, Nicholas P. Cianciotto
      Pages 151-162
    3. Klaus Heuner, Wolfgang Eisenreich
      Pages 163-181
    4. Andrew Bryan, Zachary D. Abbott, Michele S. Swanson
      Pages 197-212
    5. Emilie Portier, Yann Héchard
      Pages 213-217
    6. Jens Jäger, Michael Steinert
      Pages 225-230
  4. Cellular Microbiology of Legionella

    1. Front Matter
      Pages 231-231
    2. André N. Tiaden, Aline Kessler, Hubert Hilbi
      Pages 233-249
    3. Dennise A. De Jesús, Tamara J. O’Connor, Ralph R. Isberg
      Pages 251-264
    4. Stefan S. Weber, Annette Oxenius
      Pages 265-277
    5. Gary Faulkner, Rafael A. Garduño
      Pages 279-307
    6. Christine Hoffmann, Ivo Finsel, Hubert Hilbi
      Pages 309-321
    7. Adam J. Vogrin, Aurelie Mousnier, Gad Frankel, Elizabeth L. Hartland
      Pages 333-344
  5. Biochemical Assays to Study Legionella Effectors and Enzymes

    1. Front Matter
      Pages 345-345
    2. Stephen Weber, Stephanie Dolinsky, Hubert Hilbi
      Pages 367-380
    3. Christian Lück, Jürgen H. Helbig
      Pages 381-390
  6. Immunity and Host Response Against Legionella

    1. Front Matter
      Pages 391-391
    2. Sharon G. Berk, Rafael A. Garduño
      Pages 393-416
    3. Salvatore Bozzaro, Barbara Peracino, Ludwig Eichinger
      Pages 417-438
    4. Ann Karen C. Brassinga, Costi D. Sifri
      Pages 439-461
    5. Shandra R. Day, Costi D. Sifri, Paul S. Hoffman
      Pages 463-478
    6. Juliana I. Hori, Dario S. Zamboni
      Pages 493-503
    7. Stefan S. Weber, Nicole Joller, Annette Oxenius
      Pages 505-520
    8. Paul H. Edelstein
      Pages 521-540
    9. William R. Berrington, Thomas R. Hawn
      Pages 541-551
  7. Genomics, Transcriptomics and Proteomics of Legionella

  8. Back Matter
    Pages 603-622

About this book


Legionnaires’ disease is a severe form of pneumonia that can occur in epidemics of several hundred cases. This atypical pneumonia is characterized by a high mortality rate and affects primarily elderly and immuno-compromised individuals. An in-depth understanding of the ecology and virulence of Legionella spp. will contribute to an efficient and sustainable elimination of the bacteria from anthropogenic water systems and might lead to the development of novel therapeutics. The many different methods described in Legionella: Methods and Protocols are all specifically adapted and developed for the study of distinct features of L. pneumophila, and have already led to exciting discoveries. Most of these techniques can also be applied to the study of other bacterial pathogens, in particular intracellular pathogens like Shigella, Salmonella or Listeria. .  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Legionella: Methods and Protocols will not only be useful for research groups studying Legionella, but also for a broader scientific community studying the epidemiology, typing, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.


bacterial pathogens host pathogen interactions legionella legionella pneumophila legionnaires disease

Editors and affiliations

  • Carmen Buchrieser
    • 1
  • Hubert Hilbi
    • 2
  1. 1., Biology of Intracellular BacteriaInstitut PasteurParisFrance
  2. 2.Max von Pettenkofer InstituteLudwig-Maximilians-UniversityMunichGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-160-8
  • Online ISBN 978-1-62703-161-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site