Peroxisome Proliferator-Activated Receptors (PPARs)

Methods and Protocols

  • Mostafa Z. Badr
  • Jihan A. Youssef

Part of the Methods in Molecular Biology book series (MIMB, volume 952)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Jihan Youssef, Mostafa Z. Badr
    Pages 1-6
  3. Suong N. T. Ngo, Ross A. McKinnon
    Pages 7-34
  4. Sudhir Singh, Robert G. Bennett
    Pages 87-98
  5. Tomomi Yamazaki, Osamu Ezaki
    Pages 99-116
  6. Nico Mitro, Federica Gilardi, Marco Giudici, Cristina Godio, Elena Scotti, Maurizio Crestani
    Pages 137-144
  7. Benoit Pourcet, Bart Staels, Corine Glineur
    Pages 145-161
  8. Noor Akmal Syed Husain Sharifah, Zubaidah Zakaria, Wai Kit Chia
    Pages 187-196
  9. Aziz Guellich, Catherine Coirault
    Pages 207-217
  10. Fabio Lannutti, Alessandro Marrone, Nazzareno Re
    Pages 229-235
  11. Anna Rull, Gerard Aragonès, Raúl Beltrán-Debón, Esther Rodríguez-Gallego, Jordi Camps, Jorge Joven
    Pages 253-273

About this book

Introduction

All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity. PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, and efforts are currently underway to fully characterize their functioning and to develop safer and more effective PPAR modulators to treat a myriad of diseases and conditions. In Peroxisome proliferator-Activated Receptors: Methods and Protocols, renowned experts in the PPAR arena provide detailed protocols for investigating these receptors. Chapters contain methods ranging from the cloning of receptors to their knockdown, to protocols exploring posttranslational modifications of PPARs and coactivators, as well as receptor subcellular localization. Also assembled are methods to evaluate the involvement of these receptors in behavior functions, an emerging facet in PPAR research. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

With its well-honed methodologies, Peroxisome proliferator-Activated Receptors: Methods and Protocols,  will be a useful resource for all seeking to advance their knowledge of this field.

Keywords

PPAR activity gene targeting genotyping physiological pathways posttranslational modifications

Editors and affiliations

  • Mostafa Z. Badr
    • 1
  • Jihan A. Youssef
    • 2
  1. 1.School of PharmacyUniversity of Missouri-Kansas CityKansas CityUSA
  2. 2.School of PharmacyUniversity of Missouri-Kansas CityKansas CityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-155-4
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-154-7
  • Online ISBN 978-1-62703-155-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book