Table of contents
About this book
Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry.
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-62703-146-2
- Copyright Information Springer Science+Business Media, LLC 2013
- Publisher Name Humana Press, Totowa, NJ
- eBook Packages Springer Protocols
- Print ISBN 978-1-62703-145-5
- Online ISBN 978-1-62703-146-2
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
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