Mass Spectrometry of Glycoproteins

Methods and Protocols

  • Jennifer J. Kohler
  • Steven M. Patrie

Part of the Methods in Molecular Biology book series (MIMB, volume 951)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Steven M. Patrie, Michael J. Roth, Jennifer J. Kohler
    Pages 1-17
  3. Enrichment and Isolation Methods

    1. Front Matter
      Pages 19-19
    2. Zhi Yuan Ma, Yuliya Skorobogatko, Keith Vosseller
      Pages 21-31
    3. Andreas Hofmann, Damaris Bausch-Fluck, Bernd Wollscheid
      Pages 33-43
    4. Yawei Xu, Lijuan Zhang, Haojie Lu
      Pages 45-55
    5. Balyn W. Zaro, Howard C. Hang, Matthew R. Pratt
      Pages 57-67
  4. Sample Preparation

    1. Front Matter
      Pages 101-101
    2. Eden P. Go, Kathryn R. Rebecchi, Heather Desaire
      Pages 103-111
    3. Gerhild Zauner, Carolien A. M. Koeleman, André M. Deelder, Manfred Wuhrer
      Pages 113-127
  5. Separation Methods

    1. Front Matter
      Pages 129-129
    2. Alina D. Zamfir, Corina Flangea, Alina Serb, Ana-Maria Zagrean, Andreas M. Rizzi, Eugen Sisu
      Pages 145-169
  6. Quantitation Methods

  7. Computational Tools

    1. Front Matter
      Pages 255-255
    2. Kiyoko F. Aoki-Kinoshita
      Pages 257-267
    3. Chuan-Yih Yu, Anoop Mayampurath, Haixu Tang
      Pages 269-276
  8. Case Studies in Mass Spectrometry of Glycoproteins

    1. Front Matter
      Pages 277-277
    2. Penelope Drake, Birgit Schilling, Brad Gibson, Susan Fisher
      Pages 307-322
    3. Nicole Samuels, David Kates, Jun Liu, Joanne Severs
      Pages 323-334
  9. Back Matter
    Pages 335-339

About this book


 Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is  an essential resource for those who work at the interface of glycobiology and mass spectrometry.


Glycosylation associated ionization chromatographic strategies fragmentation methods glyco-specific computational tools glycoprotein mass spectrometry instrumentation

Editors and affiliations

  • Jennifer J. Kohler
    • 1
  • Steven M. Patrie
    • 2
  1. 1.Medical Center, Department of BiochemistryUniversity of Texas SouthwesternDallasUSA
  2. 2.Medical Center, DallasUniversity of Texas SouthwesternDallasUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-145-5
  • Online ISBN 978-1-62703-146-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site