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Nanotechnology for Nucleic Acid Delivery

Methods and Protocols

  • Manfred Ogris
  • David Oupicky

Part of the Methods in Molecular Biology book series (MIMB, volume 948)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Maria Dusinska, Zuzana Magdolenova, Lise Marie Fjellsbø
    Pages 1-12
  3. Ladan Parhamifar, Helene Andersen, S. Moein Moghimi
    Pages 13-22
  4. Masako Ichihara, Naoto Moriyoshi, Amr S. Abu Lila, Tatsuhiro Ishida, Hiroshi Kiwada
    Pages 35-47
  5. Claire Rome, Julien Gravier, Marie Morille, Gilles Divita, Anne-Laure Bolcato-Bellemin, Véronique Josserand et al.
    Pages 49-65
  6. Michel Bessodes, Nathalie Mignet
    Pages 67-84
  7. Antoine Kichler, A. James Mason, Arnaud Marquette, Burkhard Bechinger
    Pages 85-103
  8. Wolfgang Rödl, David Schaffert, Ernst Wagner, Manfred Ogris
    Pages 105-120
  9. Ye-Zi You, Jun-Jie Yan, Zhi-Qiang Yu, David Oupicky
    Pages 121-132
  10. Julia Christina Kasper, Sarah Küchler, Wolfgang Friess
    Pages 133-147
  11. Angela K. Pannier, Tatiana Segura
    Pages 149-169
  12. Eva-Christina Wurster, Asmaa Elbakry, Achim Göpferich, Miriam Breunig
    Pages 171-182
  13. Yi Zou, Lei Wan, Jenifer Blacklock, David Oupicky, Guangzhao Mao
    Pages 183-193
  14. Steven K. Cool, Bart Geers, Ine Lentacker, Stefaan C. De Smedt, Niek N. Sanders
    Pages 195-204
  15. Dialechti Vlaskou, Christian Plank, Olga Mykhaylyk
    Pages 205-241
  16. Olivia M. Merkel, Leigh M. Marsh, Holger Garn, Thomas Kissel
    Pages 263-273
  17. Xuan Jiang, Yong Ren, John-Michael Williford, Zhiping Li, Hai-Quan Mao
    Pages 275-284
  18. Back Matter
    Pages 285-286

About this book

Introduction

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Keywords

cell culture gene delivery nanoparticle delivery nucleic acid particle synthesis

Editors and affiliations

  • Manfred Ogris
    • 1
  • David Oupicky
    • 2
  1. 1., Department of PharmacyLudwig-Maximilians-University MunichMunichGermany
  2. 2., Department of Pharmaceutical SciencesWayne State UniversityDetroitUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-140-0
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-139-4
  • Online ISBN 978-1-62703-140-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site