Methods and Protocols

  • Alioscka A. Sousa
  • Michael J. Kruhlak

Part of the Methods in Molecular Biology book series (MIMB, volume 950)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Alioscka A. Sousa, Michael J. Kruhlak
    Pages 1-10
  3. Light Microscopy

    1. Front Matter
      Pages 11-11
    2. Roman Schmidt, Johann Engelhardt, Marion Lang
      Pages 27-41
    3. Yolanda Markaki, Daniel Smeets, Marion Cremer, Lothar Schermelleh
      Pages 43-64
    4. Jan Tønnesen, U. Valentin Nägerl
      Pages 65-80
    5. Dylan M. Owen, Astrid Magenau, David J. Williamson, Katharina Gaus
      Pages 81-93
    6. Grégory Giannone, Eric Hosy, Jean-Baptiste Sibarita, Daniel Choquet, Laurent Cognet
      Pages 95-110
    7. Christian Steinhauer, Michelle S. Itano, Philip Tinnefeld
      Pages 111-129
    8. Kieran Finan, Benjamin Flottmann, Mike Heilemann
      Pages 131-151
    9. Mai Yamagishi, Yoshitaka Shirasaki, Takashi Funatsu
      Pages 153-167
  4. Electron Microscopy

    1. Front Matter
      Pages 169-169
    2. Carlos Oscar Sorzano, J. M. de la Rosa Trevín, J. Otón, J. J. Vega, J. Cuenca, A. Zaldívar-Peraza et al.
      Pages 171-193
    3. Alioscka A. Sousa, Richard D. Leapman
      Pages 195-207
    4. Maria A. Aronova, Richard D. Leapman
      Pages 209-226
    5. Roman I. Koning, Abraham J. Koster
      Pages 227-251
    6. Christine L. Heinecke, Christopher J. Ackerson
      Pages 293-311
  5. Scanning Probe Microscopy

    1. Front Matter
      Pages 313-313
    2. Sergio Santos, Daniel Billingsley, Neil Thomson
      Pages 315-341
    3. Lilia A. Chtcheglova, Peter Hinterdorfer
      Pages 359-371
    4. Heath A. Huckabay, Kevin P. Armendariz, William H. Newhart, Sarah M. Wildgen, Robert C. Dunn
      Pages 373-394
  6. Complementary Techniques

    1. Front Matter
      Pages 395-395
    2. Katia Cortese, Giuseppe Vicidomini, Maria Cristina Gagliani, Patrizia Boccacci, Alberto Diaspro, Carlo Tacchetti
      Pages 417-437
    3. Dilworth Y. Parkinson, Lindsay R. Epperly, Gerry McDermott, Mark A. Le Gros, Rosanne M. Boudreau, Carolyn A. Larabell
      Pages 457-481
    4. Haley A. Klitzing, Peter K. Weber, Mary L. Kraft
      Pages 483-501
  7. Back Matter
    Pages 503-510

About this book


For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology.  Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales.  Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution.  The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography;  and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging.  Written in the successful format of the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.


electron miscroscopy high-resolution imaging macromolecular complexes mass spectrometry imaging membrane surface tomography soft x-ray tomography

Editors and affiliations

  • Alioscka A. Sousa
    • 1
  • Michael J. Kruhlak
    • 2
  1. 1.Nat. Inst. Biomedical Imaging & BioenginNational Institutes of HealthBethesdaUSA
  2. 2., National Cancer InstituteNational Institutes of HealthBethesdaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-136-3
  • Online ISBN 978-1-62703-137-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site