Epithelial Cell Culture Protocols

Second Edition

  • Scott H. Randell
  • M. Leslie Fulcher

Part of the Methods in Molecular Biology book series (MIMB, volume 945)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Sandra J. Spurr-Michaud, Ilene K. Gipson
    Pages 31-43
  3. Timothy A. Blenkinsop, Enrique Salero, Jeffrey H. Stern, Sally Temple
    Pages 45-65
  4. Kantima Leelahavanichkul, J. Silvio Gutkind
    Pages 67-79
  5. Nancy E. Rawson, Mehmet Hakan Ozdener
    Pages 81-93
  6. Mehmet Hakan Ozdener, Nancy E. Rawson
    Pages 95-107
  7. M. Leslie Fulcher, Scott H. Randell
    Pages 109-121
  8. Yingjian You, Steven L. Brody
    Pages 123-143
  9. Cathy Rasmussen, Christina Thomas-Virnig, B. Lynn Allen-Hoffmann
    Pages 161-175
  10. Luowei Li
    Pages 177-191
  11. Pierre-Alexandre Vidi, Mina J. Bissell, Sophie A. Lelièvre
    Pages 193-219
  12. Rana Mroue, Mina J. Bissell
    Pages 221-250
  13. Natalie Seach, Maree Hammett, Ann Chidgey
    Pages 251-272
  14. Maria Shulman, Yaakov Nahmias
    Pages 287-302
  15. Toshiro Sato, Hans Clevers
    Pages 319-328

About this book

Introduction

Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few. Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science.

Keywords

cell systems epithelial cells epithelial interfaces epithelial monolayers keratinocyte culture

Editors and affiliations

  • Scott H. Randell
    • 1
  • M. Leslie Fulcher
    • 2
  1. 1.Cystic Fibrosis/Pulmonary Research Ctr.University of North CarolinaChapel HillUSA
  2. 2.Cystic Fibrosis/Pulmonary Research Ctr.The University of North CarolinaChapel HillUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-125-7
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-124-0
  • Online ISBN 978-1-62703-125-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book