Fungal Secondary Metabolism

Methods and Protocols

  • Nancy P. Keller
  • Geoffrey Turner

Part of the Methods in Molecular Biology book series (MIMB, volume 944)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Kyle R. Pomraning, Kristina M. Smith, Erin L. Bredeweg, Lanelle R. Connolly, Pallavi A. Phatale, Michael Freitag
    Pages 1-22
  3. Natalie D. Fedorova, Venkatesh Moktali, Marnix H. Medema
    Pages 23-45
  4. José R. Tormo, Francisco J. Asensio, Gerald F. Bills
    Pages 59-78
  5. Gerald F. Bills, Anne W. Dombrowski, Michael A. Goetz
    Pages 79-96
  6. Christiane Wallwey, Shu-Ming Li
    Pages 121-131
  7. Hee Soo Park, Jae-Hyuk Yu
    Pages 183-190
  8. Özgür Bayram, Özlem Sarikaya Bayram, Oliver Valerius, Bastian Jöhnk, Gerhard H. Braus
    Pages 191-205
  9. Stefan Boedi, Yazmid Reyes-Dominguez, Joseph Strauss
    Pages 221-236
  10. Tanveer S. Batth, Jay D. Keasling, Christopher J. Petzold
    Pages 237-249
  11. Kwang Cheol Jeong, Jae-Hyuk Yu
    Pages 251-257
  12. Anindya Chanda, Ludmila V. Roze, John E. Linz
    Pages 259-266
  13. Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies et al.
    Pages 267-277
  14. Back Matter
    Pages 287-288

About this book


Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties. Recent focus on fungal genomics coupled with advances in detection and molecular manipulation techniques has galvanized a revitalization of this field.  Fungal Secondary Metabolism: Methods and Protocols is aimed at providing the key methodologies currently in use and necessary for accessing and exploiting the natural product information provided by the genomes of this large and varied kingdom. Written by active researchers in the field, the chapters deal with all the steps necessary, from optimization of fungal culture conditions for metabolite production, through rapid genome sequencing and bioinformatics, and genetic manipulations for functional analysis, to detection and testing of metabolites. In addition, chapters on basic science address approaches to the genetic regulation, protein biochemistry, and cellular localization of the biosynthetic pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and hands-on, Fungal Secondary Metabolism: Methods and Protocols encourages new investigators to enter the field and expands upon the expertise and range of skills of those already researching fungal natural products.


Filamentous fungi Fungal culture Fungal genomics Fungal natural products Metabolite production Secondary metabolites

Editors and affiliations

  • Nancy P. Keller
    • 1
  • Geoffrey Turner
    • 2
  1. 1., Dept. of Medical Microbiology & ImmunoloUniversity of Wisconsin-MadisonMadisonUSA
  2. 2., Dept. of Molecular Biology & BiotechnoloUniversity of SheffieldSheffieldUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-121-9
  • Online ISBN 978-1-62703-122-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site