siRNA Design

Methods and Protocols

  • Debra J. Taxman

Part of the Methods in Molecular Biology book series (MIMB, volume 942)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Yuki Naito, Kumiko Ui-Tei
    Pages 57-68
  3. Neda Nasheri, John Paul Pezacki, Selena M. Sagan
    Pages 69-86
  4. Jesper B. Bramsen, Jørgen Kjems
    Pages 87-109
  5. Elena L. Chernolovskaya, Marina A. Zenkova
    Pages 153-168
  6. Vineeth Surendranath, Mirko Theis, Bianca H. Habermann, Frank Buchholz
    Pages 193-204
  7. Luke S. Lambeth, Craig A. Smith
    Pages 205-232
  8. Ying Poi Liu, Ben Berkhout
    Pages 233-257
  9. Donald D. Rao, Neil Senzer, Zhaohui Wang, Padmasini Kumar, Chris M. Jay, John Nemunaitis
    Pages 259-278
  10. Katerina Chalupnikova, Jana Nejepinska, Petr Svoboda
    Pages 291-314
  11. Muhammad Fahim, Philip J. Larkin
    Pages 357-377
  12. Felipe Fenselau de Felippes
    Pages 379-387
  13. Back Matter
    Pages 389-392

About this book


The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi.  siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance.  The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume.  Written for the highly successful Methods in Molecular Biology™ series, this work contains the kind of detailed description and implementation advice that guarantees successful results.


Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.


RNAi Short hairpin RNA (shRNA) Small interfering RNA Therapeutic applications siRNA design strategy siRNA effector molecules siRNA target sequences

Editors and affiliations

  • Debra J. Taxman
    • 1
  1. 1., Lineberger Comprehensive Cancer CenterUniversity of North CarolinaChapel HillUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-118-9
  • Online ISBN 978-1-62703-119-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site