Methods and Protocols

  • Matt Dickinson
  • Jennifer Hodgetts

Part of the Methods in Molecular Biology book series (MIMB, volume 938)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Matt Dickinson, Melanie Tuffen, Jennifer Hodgetts
    Pages 1-14
  3. Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, Nicoletta Contaldo
    Pages 33-39
  4. Jaroslava Přibylová, Josef Špak
    Pages 41-46
  5. Heather Kingdom
    Pages 47-59
  6. Phyllis Weintraub, Jürgen Gross
    Pages 61-72
  7. Domenico Bosco, Rosemarie Tedeschi
    Pages 73-85
  8. Sabrina Bertin, Domenico Bosco
    Pages 87-108
  9. Nancy M. Andrade, Nolberto L. Arismendi
    Pages 115-121
  10. Nataša Mehle, Petra Nikolić, Matevž Rupar, Jana Boben, Maja Ravnikar, Marina Dermastia
    Pages 139-145
  11. Nigel A. Harrison, Robert E. Davis, Ericka E. Helmick
    Pages 147-158
  12. Bojan Duduk, Samanta Paltrinieri, Ing-Ming Lee, Assunta Bertaccini
    Pages 159-171
  13. Marta Martini, Ing-Ming Lee
    Pages 173-188
  14. Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Šafářová, Pavla Válová et al.
    Pages 189-204
  15. Matt Dickinson, Jennifer Hodgetts
    Pages 205-215
  16. Mogens Nicolaisen, Henriette Nyskjold, Assunta Bertaccini
    Pages 223-232
  17. Jennifer Hodgetts, Matt Dickinson
    Pages 233-244
  18. Nynne Meyn Christensen, Henriette Nyskjold, Mogens Nicolaisen
    Pages 245-252
  19. Nataša Mehle, Nina Prezelj, Matjaž Hren, Jana Boben, Kristina Gruden, Maja Ravnikar et al.
    Pages 253-268
  20. Nataša Mehle, Petra Nikolić, Kristina Gruden, Maja Ravnikar, Marina Dermastia
    Pages 269-281
  21. Olga Makarova, Nicoletta Contaldo, Samanta Paltrinieri, Assunta Bertaccini, Henriette Nyskjold, Mogens Nicolaisen
    Pages 301-317
  22. Yan Zhao, Wei Wei, Ing-Ming Lee, Jonathan Shao, Xiaobing Suo, Robert E. Davis
    Pages 329-338
  23. Xianling Ji, Yingping Gai
    Pages 339-349
  24. Luciana Galetto, Majid Siampour, Cristina Marzachì
    Pages 351-369
  25. Mark T. Andersen, Lia W. Liefting
    Pages 371-379
  26. Lucy T. T. Tran-Nguyen, Bernd Schneider
    Pages 381-393
  27. Sylvie Malembic-Maher, Patricia Carle
    Pages 405-416
  28. Back Matter
    Pages 417-421

About this book


Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.


phytoplasma diseases phytoplasma genomes plant DNA plant pathology taxonomic groups

Editors and affiliations

  • Matt Dickinson
    • 1
  • Jennifer Hodgetts
    • 2
  1. 1.School of BiosciencesThe University of NottinghamNottinghamUnited Kingdom
  2. 2.The Food and Environment Research AgencySand HuttonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-088-5
  • Online ISBN 978-1-62703-089-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site