Table of contents

  1. Front Matter
    Pages i-xi
  2. General

    1. Front Matter
      Pages 1-1
    2. Anish Patel, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, David G. Lambert
      Pages 37-47
  3. Specialist Measurement Systems

    1. Front Matter
      Pages 49-49
    2. Stephen C. Tovey, Paul J. Brighton, Edward T. W. Bampton, Yan Huang, Gary B. Willars
      Pages 51-93
    3. Ian C. B. Marshall, Izzy Boyfield, Shaun McNulty
      Pages 95-101
    4. Ian C. B. Marshall, Davina E. Owen, Shaun McNulty
      Pages 103-109
  4. Measurement of Ca2+ Channel Activity

  5. Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores

    1. Front Matter
      Pages 161-161
  6. Measurement of Ins(1,4,5)P3 and Ca2+ Release Form Intracellular Stores

  7. Specialist Measurement Techniques

About this book

Introduction

The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.  With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes.  Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.

Keywords

Calcium signaling Channel activity measurement Intracellular Ca2+ Regulation Specialist measurement systems Store release Suspension-based fluorimetric assay

Editors and affiliations

  • David G. Lambert
    • 1
  • Richard D. Rainbow
    • 2
  1. 1., Department of Cardiovascular SciencesLeicester Royal InfirmaryLeicesterUnited Kingdom
  2. 2., Department of Cardiovascular SciencesLeicester Royal InfirmaryLeicesterUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-086-1
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-085-4
  • Online ISBN 978-1-62703-086-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book