Table of contents

  1. Front Matter
    Pages i-xi
  2. Shao-Yao Ying, Donald C. Chang, Shi-Lung Lin
    Pages 1-19
  3. Julia Starega-Roslan, Wlodzimierz J. Krzyzosiak
    Pages 21-34
  4. Anna Majer, Kyle A. Caligiuri, Stephanie A. Booth
    Pages 35-45
  5. Ludwig Christian Hinske, Jens Heyn, Pedro A. F. Galante, Lucila Ohno-Machado, Simone Kreth
    Pages 69-76
  6. Chih-Hao Kuo, Mark D. Goldberg, Shi-Lung Lin, Shao-Yao Ying, Jiang F. Zhong
    Pages 77-82
  7. Jens Heyn, Ludwig Christian Hinske, Carola Ledderose, Elisabeth Limbeck, Simone Kreth
    Pages 83-90
  8. Jianzhen Xu, Chi-Wai Wong
    Pages 91-103
  9. Yi Jin, Zujian Chen, Xiqiang Liu, Xiaofeng Zhou
    Pages 117-127
  10. Meltem Isik, Eugene Berezikov
    Pages 129-141
  11. Giovanni Lugli, Neil R. Smalheiser
    Pages 173-179
  12. Scott L. Pratt, T. Ashley Burns, Marcy D. Owens, Susan K. Duckett
    Pages 181-194
  13. Ping Wu, Melissa A. Wilmarth, Feng Zhang, Guangwei Du
    Pages 195-207
  14. Shi-Lung Lin, Shao-Yao Ying
    Pages 209-229
  15. Angeliki Pantazi, Athanasios Zovoilis
    Pages 231-245
  16. Shi-Lung Lin, Donald C. Chang, Shao-Yao Ying
    Pages 271-278
  17. Shi-Lung Lin, Shin-Ju E. Chang, Shao-Yao Ying
    Pages 279-294
  18. Janice M. Yoshizawa, David T. W. Wong
    Pages 313-324
  19. Jack S. K. Chen, David T. S. Wu
    Pages 325-341
  20. Jaideep Banerjee, Chandan K. Sen
    Pages 343-356
  21. Jia Han Deng, Qinggao Deng, Chih-Hao Kuo, Sean W. Delaney, Shao-Yao Ying
    Pages 357-369
  22. Back Matter
    Pages 371-375

About this book


Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity; yet many questions still remain.  In MicroRNA Protocols, Second Edition, experts in the field provide up-to-date coverage of this diverse area of study.  The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and known pitfalls.


Fully updated and easy to use, MicroRNA Protocols, Second Edition aims to stimulate readers to explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields.


Drug agent development Expression profiling Gene expression regulation Target validation miRNA

Editors and affiliations

  • Shao-Yao Ying
    • 1
  1. 1.Keck School of Medicine, Dept. of Cell and NeurobiologyUniversity of Southern CaliforniaLos AngelesUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-082-3
  • Online ISBN 978-1-62703-083-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site