Methods and Protocols

  • Douglas T. Carrell
  • Kenneth I. Aston

Part of the Methods in Molecular Biology book series (MIMB, volume 927)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Semen Analysis Methods

    1. Front Matter
      Pages 1-1
    2. Sergey I. Moskovtsev, Clifford L. Librach
      Pages 13-19
    3. Sivakumar Ramu, Rajasingam S. Jeyendran
      Pages 21-25
    4. Susan A. Rothmann, Anna-Marie Bort, John Quigley, Robin Pillow
      Pages 27-37
    5. Hiroaki Shibahara, Junko Koriyama
      Pages 51-60
    6. David Mortimer, Sharon T. Mortimer
      Pages 61-75
  3. Tests of Sperm Function

    1. Front Matter
      Pages 89-89
    2. Sergio Oehninger, Mahmood Morshedi, Daniel Franken
      Pages 91-102
    3. Kathleen Hwang, Dolores J. Lamb
      Pages 103-111
  4. Sperm DNA Damage Testing

    1. Front Matter
      Pages 119-119
    2. Rakesh Sharma, Jayson Masaki, Ashok Agarwal
      Pages 121-136
    3. Luke Simon, Douglas T. Carrell
      Pages 137-146
    4. Donald P. Evenson
      Pages 147-164
  5. Advanced Clinical Testing

  6. Sperm Preparation and Selection Techniques

    1. Front Matter
      Pages 207-207
    2. Tiffany Justice, Greg Christensen
      Pages 209-215
    3. David Mortimer, Sharon T. Mortimer
      Pages 217-226
    4. Shilo L. Archer, William E. Roudebush
      Pages 241-245
    5. Luke Simon, Aaron Wilcox, Douglas T. Carrell
      Pages 247-256
    6. Sonja Grunewald, Uwe Paasch
      Pages 257-262
    7. Mohammad Hossein Nasr-Esfahani, Tavalaee Marziyeh
      Pages 263-268
    8. Luke Simon, Shao-qin Ge, Douglas T. Carrell
      Pages 269-278
    9. Duane L. Garner, K. Michael Evans, George E. Seidel
      Pages 279-295
  7. Staging and Histology Techniques

    1. Front Matter
      Pages 297-297
    2. Marvin L. Meistrich, Rex A. Hess
      Pages 299-307
    3. Cathryn A. Hogarth, Michael D. Griswold
      Pages 309-320
  8. Oxidative Stress Testing

    1. Front Matter
      Pages 349-349
    2. Anthony H. Kashou, Rakesh Sharma, Ashok Agarwal
      Pages 351-361
    3. Charles H. Muller, Tiffany K. Y. Lee, Michalina A. Montaño
      Pages 363-376
  9. Analytical Tools and Methods

    1. Front Matter
      Pages 377-377
    2. Robert J. Goodrich, Ester Anton, Stephen A. Krawetz
      Pages 385-396
    3. Sara de Mateo, Josep Maria Estanyol, Rafael Oliva
      Pages 411-422
  10. Methodologies to Evaluate Sperm Epigenetics

About this book


Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.


clinical andrology semen analysis count sperm DNA damage sperm epigenetics sperm function

Editors and affiliations

  • Douglas T. Carrell
    • 1
  • Kenneth I. Aston
    • 2
  1. 1.Andrology and IVF Laboratories, Department of Surgery (Urology)University of Utah School of MedicineSalt Lake CityUSA
  2. 2., Andrology and IVF LaboratoriesUniversity of Utah School of MedicineSalt Lake CityUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-037-3
  • Online ISBN 978-1-62703-038-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site