Methods and Protocols

  • Robert Ménard

Part of the Methods in Molecular Biology book series (MIMB, volume 923)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Parasite Culturing

    1. Front Matter
      Pages 1-1
    2. Alexander G. Maier, Melanie Rug
      Pages 3-15
    3. Kathryn Shaw Saliba, Marcelo Jacobs-Lorena
      Pages 17-25
    4. Anil K. Ghosh, Marcelo Jacobs-Lorena
      Pages 27-33
    5. Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, Clemens H. M. Kocken
      Pages 35-49
    6. Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker et al.
      Pages 51-72
  3. Genome Manipulation

    1. Front Matter
      Pages 73-73
    2. Melanie Rug, Alexander G. Maier
      Pages 75-98
    3. Nisha Philip, Rachael Orr, Andrew P. Waters
      Pages 99-125
    4. Claudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, Oliver Billker
      Pages 127-138
    5. Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, Chris J. Janse
      Pages 139-150
    6. Cecilia P. Sanchez, Judith Pfahler, Hernando A. del Portillo, Michael Lanzer
      Pages 151-159
    7. Marina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, Stéphanie A. Blandin
      Pages 161-176
  4. “OMIC” Techniques

    1. Front Matter
      Pages 177-177
    2. Sohini Sanyal, Thomas J. Templeton, Cristina K. Moreira
      Pages 179-188
    3. Zbynek Bozdech, Sachel Mok, Archna P. Gupta
      Pages 189-211
    4. Heather J. Painter, Lindsey M. Altenhofen, Björn F. C. Kafsack, Manuel Llinás
      Pages 213-219
    5. Wieteke A. M. Hoeijmakers, Richárd Bártfai, Hendrik G. Stunnenberg
      Pages 221-239
    6. Dominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, Christian Doerig
      Pages 241-257
  5. Red Blood Cell Stages

    1. Front Matter
      Pages 267-267
    2. Catherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon et al.
      Pages 291-297
    3. Anand Srivastava, Yves Durocher, Benoît Gamain
      Pages 307-319
    4. Jose-Juan Lopez-Rubio, T. Nicolai Siegel, Artur Scherf
      Pages 321-333
    5. Joanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, Blandine Franke-Fayard
      Pages 353-368
  6. Pre-Erythrocytic Stages

    1. Front Matter
      Pages 369-369
    2. Allison F. Carey, Robert Ménard, Daniel Y. Bargieri
      Pages 371-383
    3. Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, Maria M. Mota
      Pages 385-400
    4. Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, Rogerio Amino
      Pages 401-410
    5. Rebecca R. Stanway, Jacqueline Schmuckli-Maurer, Volker T. Heussler
      Pages 411-427
    6. Takeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, Shahid M. Khan
      Pages 429-443
  7. Immunity to Plasmodium

  8. Controlling Plasmodium Infection

    1. Front Matter
      Pages 505-505
    2. Jing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, Blandine Franke-Fayard
      Pages 507-522
    3. Anthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, Jean-Christophe Barale
      Pages 523-534
    4. Ashley M. Vaughan, Stefan H. I. Kappe
      Pages 549-566
    5. Laurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, Georges Snounou
      Pages 567-576
    6. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, Robert E. Sinden
      Pages 577-600
    7. Silke Fuchs, Tony Nolan, Andrea Crisanti
      Pages 601-622
  9. Back Matter
    Pages 623-626

About this book


Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of  Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, "omic" approaches, and  techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical,  Malaria: Methods and Protocols, Second Edition offers a comprehensive set of standard techniques for laboratory  researchers.


Plasmodium cellular malariologists culture techniques genome manipulation molecular malariologists parasite culture techniques parasite genome ‘omic’ approaches

Editors and affiliations

  • Robert Ménard
    • 1
  1. 1., Malaria Biology and Genetics UnitInstitut PasteurParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-025-0
  • Online ISBN 978-1-62703-026-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site