Genomic Imprinting

Methods and Protocols

  • Nora Engel

Part of the Methods in Molecular Biology book series (MIMB, volume 925)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Parent-of-Origin Effects

    1. Front Matter
      Pages 1-1
    2. Yong Cheng, Dasari Amarnath, Keith E. Latham
      Pages 3-19
    3. Masanori Imamura, Hironobu Okuno, Ikuo Tomioka, Yoshimi Kawamura, Zachary Yu-Ching Lin, Ryusuke Nakajima et al.
      Pages 21-48
    4. Michael C. Golding
      Pages 49-59
    5. Emily Y. Smith, James L. Resnick
      Pages 61-66
  3. Identifying Imprinted Genes

  4. Identifying the Regulatory Features of Imprinted Domains

    1. Front Matter
      Pages 135-135
  5. Epigenetics of Imprinted Regions

    1. Front Matter
      Pages 147-147
    2. Julie Borgel, Sylvain Guibert, Michael Weber
      Pages 149-158
    3. Raffaella Nativio, Yoko Ito, Adele Murrell
      Pages 173-185
  6. Analysis of Imprinted Expression

    1. Front Matter
      Pages 199-199
    2. Victoria A. Moran, Courtney N. Niland, Ahmad M. Khalil
      Pages 219-228
  7. Imprinting in Plants

    1. Front Matter
      Pages 229-229
  8. Evolution of Imprinted Genes

    1. Front Matter
      Pages 249-249
    2. Martina Paulsen
      Pages 251-262
    3. Elena de la Casa-Esperón
      Pages 277-294
  9. Back Matter
    Pages 295-297

About this book


Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting.  This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in  unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.


Bioinformatics Epigenetic profiles Gene regulation Genomic evolution Imprinted genes Parent-of-origin effects Regulatory feature identification

Editors and affiliations

  • Nora Engel
    • 1
  1. 1.School of Medicine, Fels InstituteTemple UniversityPhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-010-6
  • Online ISBN 978-1-62703-011-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site