DNA Repair Protocols

  • Lotte Bjergbæk
Part of the Methods in Molecular Biology book series (MIMB, volume 920)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Identification of Repair Genes and Generation of Repair Mutants

    1. Front Matter
      Pages 7-7
    2. Lone Vedel Schøler, Tine Hørning Møller, Steffen Nørgaard, Lotte Vestergaard, Anders Olsen
      Pages 27-38
    3. Masamichi Ishiai, Emi Uchida, Minoru Takata
      Pages 39-49
  3. Assessing DNA Damage and Repair

    1. Front Matter
      Pages 51-51
    2. Chun Tsai, Vaughn Smider, Byung Joon Hwang, Gilbert Chu
      Pages 53-78
    3. Wiebke Schlörmann, Michael Glei
      Pages 91-100
    4. Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, Bennett Van Houten
      Pages 111-132
  4. Repair of Incorrect or Damaged DNA Bases

    1. Front Matter
      Pages 133-133
    2. Liya Gu, Charles M. Ensor, Guo-Min Li
      Pages 135-147
    3. Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, Yanbin Zhang
      Pages 149-162
    4. Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, Marcus S. Cooke
      Pages 163-175
    5. Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, Orlando D. Schärer
      Pages 203-219
    6. Puck Knipscheer, Markus Räschle, Orlando D. Schärer, Johannes C. Walter
      Pages 221-243

About this book

Introduction

Current knowledge of the mechanisms that regulate DNA repair has grown significantly over the past years with technology advances such as RNA interference, advanced proteomics and microscopy as well as high throughput screens. The third  edition of DNA Repair Protocols covers various aspects of the eukaryotic response to genomic insult including recent advanced protocols as well as standard techniques used in the field of DNA repair. Both mammalian and non-mammalian model organisms are covered in the book, and many of the techniques can be applied with only minor modifications to other systems than the one described. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

 

Thorough and intuitive, DNA Repair Protocols, Third Edition provides expert guidance for DNA repair, recombination, and replication.

Keywords

Arabidopsis cell extracts DNA repair RNA Saccharomyces cerevisiae chromosomal DNA electrophoretic mobility shift microgel eletrophoresis techinque plasmid DNA repair genes yeast cells

Editors and affiliations

  • Lotte Bjergbæk
    • 1
  1. 1., Department of Molecular BiologyAarhus UniversityÅrhus CDenmark

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-998-3
  • Copyright Information Springer Science+Business Media New York 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-997-6
  • Online ISBN 978-1-61779-998-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book