Bacterial Regulatory RNA

Methods and Protocols

  • Kenneth C. Keiler

Part of the Methods in Molecular Biology book series (MIMB, volume 905)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Identification of Regulatory RNAs

    1. Front Matter
      Pages 1-1
    2. Christine Drevet, Christine Pourcel
      Pages 15-27
  3. Function and Expression of Regulatory RNAs

  4. RNA Structure and Biophysical Measurements

    1. Front Matter
      Pages 97-97
    2. Matthew G. Seetin, David H. Mathews
      Pages 99-122
  5. RNA–Protein Interactions

    1. Front Matter
      Pages 175-175
    2. Colin P. Corcoran, Renate Rieder, Dimitri Podkaminski, Benjamin Hofmann, Jörg Vogel
      Pages 177-200
    3. Alexander V. Yakhnin, Helen Yakhnin, Paul Babitzke
      Pages 201-211
    4. Yi Peng, Toby J. Soper, Sarah A. Woodson
      Pages 213-224
  6. RNA–RNA Interactions

    1. Front Matter
      Pages 225-225
    2. Teppei Morita, Kimika Maki, Hiroji Aiba
      Pages 235-244
    3. Guillaume Desnoyers, Eric Massé
      Pages 245-255
  7. Interactions of Regulatory RNA with RNA Polymerase and the Ribosome

    1. Front Matter
      Pages 257-257
    2. Preeti Mehta, Perry Woo, Krithika Venkataraman, A. Wali Karzai
      Pages 273-289
    3. Brian D. Janssen, Elie J. Diner, Christopher S. Hayes
      Pages 291-309
    4. Daisuke Kurita, Akira Muto, Hyouta Himeno
      Pages 311-325
  8. Back Matter
    Pages 327-333

About this book


The discovery of wide-spread RNA-based regulation in bacteria has led to new evaluations of the importance of bacterial regulatory RNA in every aspect of bacterial physiology. In Bacteria Regulatory RNA: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study bacterial regulatory RNA. These include methods and techniques to identify regulatory RNAs, characterizing the function and expression of regulatory RNAs in bacterial cells, RNA structure prediction, and interactions between regulatory RNAs and proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


 Authoritative and practical, Bacteria Regulatory RNA: Methods and Protocols seeks to aid scientists in the further study of bacterial regulatory RNA.


RNA RNA polymerase bacterial cells biofilm formation developmental programs genomic approaches growth regulation pathogenesis quorum sensing ribosome

Editors and affiliations

  • Kenneth C. Keiler
    • 1
  1. 1., Dept. of Biochemistry and Molecular BiolThe Pennsylvania State UniversityUniversity ParkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-948-8
  • Online ISBN 978-1-61779-949-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site