Diagnosis of Sexually Transmitted Diseases

Methods and Protocols

  • Colin R. MacKenzie
  • Birgit Henrich

Part of the Methods in Molecular Biology book series (MIMB, volume 903)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Fernando Vazquez, Luis Otero, Santiago Melón, María de Oña
    Pages 1-20
  3. Davide Gibellini, Elisa De Crignis, Maria Carla Re
    Pages 21-34
  4. Cheng-Yen Chen, Ronald C. Ballard
    Pages 103-112
  5. Jutte J. C. de Vries, Maria Barbi, Sandro Binda, Eric C. J. Claas
    Pages 169-175
  6. Seweryn Bialasiewicz, Ian M. Mackay, David M. Whiley, Theo P. Sloots
    Pages 225-233
  7. Nadine Möbius, Wibke Brenneisen, Anke Schaeffer, Birgit Henrich
    Pages 235-253

About this book

Introduction

Sexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world. Human immunodeficiency virus infections and the ensuing opportunistic infections are a major drain on the human and financial resources of many countries in the developing world and even with the availability of effective treatment the epidemic is not yet contained. Screening in developing worlds is difficult and there is a need for simple reliable cheap diagnostic methods that can be performed at the point of care, on the same day and by staff with limited training. Molecular biological methods are very attractive for the diagnosis of STI since a well defined range of pathogens is responsible for the infection. Diagnosis of Sexually Transmitted Diseases: Methods and Protocols strives to cover the full range of molecular testing for STI. Chapters cover a variety of topics such as aspects of DNA extraction from small volume samples or difficult tissues, simple, nested or multiplex PCR, use of duplex primers or other modifications of primers and PCR conditions, sequence analysis for genotyping, denaturing gel analysis, microarrays using liquid beads or microspheres and silicon nanoparticle-enhanced microcantilever detection of DNA. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Diagnosis of Sexually Transmitted Diseases: Methods and Protocols will be a valuable reference for the scientist looking to establish molecular methodologies for the diagnosis of STI tests in their own lab.

Keywords

PCR methods immunodeficiency virus infections molecular biological methods sexually transmitted disease (STD) sexually transmitted infection (STI)

Editors and affiliations

  • Colin R. MacKenzie
    • 1
  • Birgit Henrich
    • 2
  1. 1.University Hospital, Institute of Medical MicrobiologyHeinrich-Heine-UniversityDüsseldorfGermany
  2. 2.University Hospital, Institute of Medical MicrobiologyHeinrich-Heine-UniversityDüsseldorfGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-937-2
  • Copyright Information Springer Science+Business Media New York 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-936-5
  • Online ISBN 978-1-61779-937-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book