Microbial Carotenoids From Fungi

Methods and Protocols

  • José-Luis Barredo

Part of the Methods in Molecular Biology book series (MIMB, volume 898)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Arnold L. Demain, José L. Adrio
    Pages 1-40
  3. Preejith Vachali, Prakash Bhosale, Paul S. Bernstein
    Pages 41-59
  4. Santiago Torres-Martínez, Rosa M. Ruiz-Vázquez, Victoriano Garre, Sergio López-García, Eusebio Navarro, Ana Vila
    Pages 85-107
  5. Enrique A. Iturriaga, Tamás Papp, María Isabel Álvarez, Arturo P. Eslava
    Pages 109-122
  6. Tamás Papp, Árpád Csernetics, Ildikó Nyilasi, Csaba Vágvölgyi, Enrique A. Iturriaga
    Pages 123-132
  7. Marta Rodríguez-Sáiz, Juan-Luis de la Fuente, José-Luis Barredo
    Pages 133-151
  8. Rick W. Ye, Pamela L. Sharpe, Quinn Zhu
    Pages 153-159
  9. Diego Libkind, Martín Moliné, Celia Tognetti
    Pages 183-194
  10. Marcelo Baeza, María Fernández-Lobato, Víctor Cifuentes
    Pages 195-205
  11. Mauricio Niklitschek, Marcelo Baeza, María Fernández-Lobato, Víctor Cifuentes
    Pages 219-234
  12. Guangyun Lin, Joanna Bultman, Eric A. Johnson, Jack W. Fell
    Pages 235-249
  13. Javier Avalos, Alfonso Prado-Cabrero, Alejandro F. Estrada
    Pages 263-274
  14. Martín Moliné, Diego Libkind, María van Broock
    Pages 275-283
  15. Back Matter
    Pages 285-290

About this book


Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage, and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids From Fungi: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the manipulation and metabolic engineering of the Beta-carotene producing fungi Blakeslea trispora and Mucor circinelloides, lycopene production with the yeast Yarrowia lipolytica, peroxisome targeting of lycopene pathway enzymes in Pichia pastoris, and the manipulation of the heterobasidiomycetous yeast Xanthophyllomyces dendrorhous, which produces astaxanthin, a red xanthophyll with large importance in the aquaculture, pharmaceutical, and food industries. Additionally, the book includes a DNA assembler method for construction of zeaxanthin-producing strains of Saccharomyces cerevisiae, production of neurosporaxanthin by Neurospora and Fusarium, and production of torularhodin, torulene and b-carotene by Rhodotorula yeasts. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microbial Carotenoids From Fungi: Methods and Protocols, provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms.


Blakeslea trispora Carotenoids DNA assembler Mucor circinelloides Saccharomyces cerevisiae Xanthophyllomyces dendrorhous Yarrowia lipolytica biosynthetic pathways biotechnological processes fungi photosynthetic organisms

Editors and affiliations

  • José-Luis Barredo
    • 1
  1. 1.Parque Tecnológico de LeónGadea BiopharmaValladolidSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-917-4
  • Online ISBN 978-1-61779-918-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site