Quantitative Methods in Proteomics

  • Katrin Marcus

Part of the Methods in Molecular Biology book series (MIMB, volume 893)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Planning a Proteomic Experiment

    1. Front Matter
      Pages 1-1
  3. Quantitative Proteomics: Gel-Based Methods

    1. Front Matter
      Pages 23-23
    2. Caroline May, Frederic Brosseron, Kathy Pfeiffer, Helmut E. Meyer, Katrin Marcus
      Pages 37-46
    3. Nadine Dyballa, Sabine Metzger
      Pages 47-59
    4. Thierry Rabilloud
      Pages 61-73
    5. Caroline May, Frederic Brosseron, Piotr Chartowski, Helmut E. Meyer, Katrin Marcus
      Pages 75-82
  4. Quantitative Proteomics: MS-Based Methods

    1. Front Matter
      Pages 83-83
    2. Miroslav Nikolov, Carla Schmidt, Henning Urlaub
      Pages 85-100
    3. Florian Beck, Julia Maria Burkhart, Joerg Geiger, René Peiman Zahedi, Albert Sickmann
      Pages 101-113
    4. Christian J. Koehler, Magnus Ø. Arntzen, Achim Treumann, Bernd Thiede
      Pages 129-141
    5. Josef Kellermann, Friedrich Lottspeich
      Pages 143-153
    6. Heike Piechura, Silke Oeljeklaus, Bettina Warscheid
      Pages 201-221
    7. Xiaoying Ye, Brian T. Luke, Donald J. Johann Jr, King C. Chan, DaRue A. Prieto, Akira Ono et al.
      Pages 223-240
    8. Barbara Sitek, Daniel M. Waldera-Lupa, Gereon Poschmann, Helmut E. Meyer, Kai Stühler
      Pages 241-248
    9. Philip J. Brownridge, Victoria M. Harman, Deborah M. Simpson, Robert J. Beynon
      Pages 267-293
    10. Sasha Singh, Marc Kirchner, Judith A. Steen, Hanno Steen
      Pages 295-319
    11. Christine Vogel, Edward M. Marcotte
      Pages 321-341
  5. Specific Methods–Detection and Quantification of Post-translational Modifications

    1. Front Matter
      Pages 343-343
    2. Stefan Helling, Maik Hüttemann, Bernhard Kadenbach, Rabia Ramzan, Sebastian Vogt, Katrin Marcus
      Pages 345-358
    3. Yadong Huang, Jay J. Thelen
      Pages 359-370
    4. L. Renee Ruhaak, Carolin Huhn, Carolien A. M. Koeleman, André M. Deelder, Manfred Wuhrer
      Pages 371-385
    5. Claudia Lindemann, Lars I. Leichert
      Pages 387-403
    6. Federico Torta, Angela Bachi
      Pages 405-416
    7. He-Hsuan Hsiao, Erik Meulmeester, Henning Urlaub
      Pages 431-441
  6. Data Analysis and Software Tools

    1. Front Matter
      Pages 443-443
    2. Martin Eisenacher, Michael Kohl, Michael Turewicz, Markus-Hermann Koch, Julian Uszkoreit, Christian Stephan
      Pages 445-488
    3. Simone Lemeer, Hannes Hahne, Fiona Pachl, Bernhard Kuster
      Pages 489-499
    4. Marc Vaudel, Julia Maria Burkhart, René Peiman Zahedi, Lennart Martens, Albert Sickmann
      Pages 501-509
    5. Joost W. Gouw, Jeroen Krijgsveld
      Pages 511-522
  7. Back Matter
    Pages 523-539

About this book


Protein modifications and changes made to them, as well as the quantities of expressed proteins, can define the various functional stages of the cell. Accordingly, perturbations can lead to various diseases and disorders. As a result, it has become paramount to be able to detect and monitor post-translational modifications and to measure the abundance of proteins within the cell with extreme sensitivity. While protein identification is an almost routine requirement nowadays, reliable techniques for quantifying unmodified proteins (including those that escape detection under standard conditions, such as protein isoforms and membrane proteins) is not routine. Quantitative Methods in Proteomics gives a detailed survey of topics and methods on the principles underlying modern protein analysis, from statistical issues when planning proteomics experiments, to gel-based and mass spectrometry-based applications. The quantification of post-translational modifications is also addressed, followed by the “hot” topics of software and data analysis, as well as various overview chapters which provide a comprehensive overview of existing methods in quantitative proteomics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Quantitative Methods in Proteomics serves as a comprehensive and competent overview of the important and still growing field of quantitative proteomics.


MS-based methods enzymatic labeling isotope labeling membrane proteins metabolic labeling protein isoforms translational modifications

Editors and affiliations

  • Katrin Marcus
    • 1
  1. 1.Fak. Medizin, Medizinische Proteom CenterUniversität BochumBochumGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-884-9
  • Online ISBN 978-1-61779-885-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site