Microbial Carotenoids from Bacteria and Microalgae

Methods and Protocols

  • José-Luis Barredo

Part of the Methods in Molecular Biology book series (MIMB, volume 892)

Table of contents

  1. Front Matter
    Pages i-xi
  2. J. Paniagua-Michel, Jorge Olmos-Soto, Manuel Acosta Ruiz
    Pages 1-12
  3. Francisco Romero, Rosa Isabel Fernández-Chimeno, Juan Luis de la Fuente, José-Luis Barredo
    Pages 13-20
  4. Dalal Asker, Tarek S. Awad, Teruhiko Beppu, Kenji Ueda
    Pages 21-60
  5. Dalal Asker, Tarek S. Awad, Teruhiko Beppu, Kenji Ueda
    Pages 99-131
  6. Patricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, Tomás G. Villa
    Pages 159-172
  7. María de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García, Encarnación Mellado
    Pages 207-217
  8. Mark A. Scaife, Cynthia A. Ma, Phillip C. Wright, Roberto E. Armenta
    Pages 255-268
  9. Marta Vila, Aurora Galván, Emilio Fernández, Rosa León
    Pages 283-295
  10. Hajime Masukawa, Mari Mochimaru, Shinichi Takaichi
    Pages 297-306
  11. José M. Fernández-Sevilla, F. Gabriel Acién Fernández, Emilio Molina Grima
    Pages 307-314
  12. Eva Eylert, Adelbert Bacher, Wolfgang Eisenreich
    Pages 315-333
  13. Laura Pérez-Fons, Paul D. Fraser
    Pages 335-345
  14. Back Matter
    Pages 347-355

About this book


Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the study of canthaxanthin production, construction of carotenoid reporter systems, directed evolution of carotenoid synthases, and improvement of b-carotene hydroxylase catalytic activity are described. Additionally, the book includes methods of DNA fingerprinting for the identification of carotenogenic Dunaliella species, ketocarotenoid biosynthesis in microalgae expressing the beta-C-4-carotene oxygenase gene, characterization of carotenogenesis genes in Anabaena sp., obtaining lutein from microalgal biomass, NMR-based isotopologue profiling of microbial carotenoids, and analysis of diapocarotenoids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms.


Blakeslea trispora Carotenoids Dunaliella salina Haematococcus pluvialis Xanthophyllomyces dendrorhous algae microorganisms production

Editors and affiliations

  • José-Luis Barredo
    • 1
  1. 1.Parque Tecnológico de LeónGadea BiopharmaValladolidSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-878-8
  • Online ISBN 978-1-61779-879-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site