Kidney Development

Methods and Protocols

  • Odyssé Michos

Part of the Methods in Molecular Biology™ book series (MIMB, volume 886)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Organ Culture, Tissue Manipulation, and Live Imaging

    1. Front Matter
      Pages 1-1
    2. Paul N. Riccio, Odyssé Michos
      Pages 3-11
    3. Xing Zhang, Kevin T. Bush, Sanjay K. Nigam
      Pages 13-21
    4. David Grote, Michael Marcotte, Maxime Bouchard
      Pages 31-43
    5. Ekaterina Batourina, Devangini Gandhi, Cathy L. Mendelsohn, Andrei Molotkov
      Pages 45-53
    6. Aleksandr Vasilyev, Iain A. Drummond
      Pages 55-70
  3. Analyzing Ureteric Bud Branching and Nephrogenesis

    1. Front Matter
      Pages 71-71
    2. Kieran M. Short, Ian M. Smyth
      Pages 95-107
    3. Luise A. Cullen-McEwen, James A. Armitage, Jens R. Nyengaard, John F. Bertram
      Pages 109-119
  4. Renal Primary Cell Cultures and Cell Lines

    1. Front Matter
      Pages 133-133
    2. Jamie A. Davies, Mathieu Unbekandt, Jessica Ineson, Michael Lusis, Melissa H. Little
      Pages 135-146
    3. Guanping Tai, Peter Hohenstein, Jamie A. Davies
      Pages 165-171
    4. Sriram Manivannan, Jason P. Gleghorn, Celeste M. Nelson
      Pages 173-182
  5. Detecting Gene/Protein Expression and Signaling

    1. Front Matter
      Pages 183-183
    2. Jamie A. Davies, Melissa H. Little, Bruce Aronow, Jane Armstrong, Jane Brennan, Sue Lloyd-MacGilp et al.
      Pages 185-201
    3. Shifaan Thowfeequ, Odyssé Michos
      Pages 203-210
    4. S. Steven Potter, Eric W. Brunskill
      Pages 211-221
    5. Bree A. Rumballe, Han Sheng Chiu, Kylie M. Georgas, Melissa H. Little
      Pages 223-239
  6. Manipulating Gene Expression in Developing and Adult Kidney

    1. Front Matter
      Pages 293-293
    2. Jamie A. Davies, Mathieu Unbekandt
      Pages 295-303
    3. Cristina Cebrián
      Pages 311-319
    4. Guadalupe Ortiz-Muñoz, Beñat Mallavia, Oscar Lopez-Franco, Purificacion Hernandez-Vargas, Jesus Egido, Carmen Gomez-Guerrero
      Pages 321-329
  7. Analyzing Functional Defects of the Kidney and Urinary Tract

    1. Front Matter
      Pages 331-331
    2. Luise A. Cullen-McEwen, Rebecca N. Douglas-Denton, John F. Bertram
      Pages 333-350
    3. Leif Oxburgh, Mark P. de Caestecker
      Pages 363-379
    4. Ashley R. Carpenter, Brian Becknell, Daniel A. Hirselj, Kirk M. McHugh
      Pages 393-402
    5. Ashley R. Carpenter, Brian Becknell, Susan E. Ingraham, Kirk M. McHugh
      Pages 403-410
  8. Jamie A. Davies, Melissa H. Little, Bruce Aronow, Jane Armstrong, Jane Brennan, Sue Lloyd-MacGilp et al.
    Pages E1-E1
  9. Back Matter
    Pages 411-415

About this book


Over the last decade the development of new molecular biology tools, advanced microscopy, live imaging and systems biology approaches have revolutionized our conception of how embryonic development proceeds.  One fundamental aspect of development biology is the concept of morphogenesis: understanding how a group of multipotent cells organize and differentiate into a complex organ.  In Kidney Development: Methods and Protocols, expert researchers in the field detail different approaches to tackle kidney development. These approaches include culture and live imaging aspects of kidney development, analyzing the 3-dimensional aspects of branching morphogenesis as well as nephrogenesis, manipulation of the gene/protein expression during kidney development as well as in the adult kidney, and how to assess kidney malformation and disease. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Kidney Development: Methods and Protocols seeks to aid scientists in the further study of the process of morphogenesis which is fundamental important not only for studying developmental biology but also for regenerative medicine.


branching morphogenesis cell migration cell signaling disease ex vivo culture kidney development kidney malformation kidney organogenesis nephrogenesis

Editors and affiliations

  • Odyssé Michos
    • 1
  1. 1.Hammer Health Sciences Center Department of Genetics and DevelopmentColumbia University Medical CenterNew YorkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-850-4
  • Online ISBN 978-1-61779-851-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site