Retinal Development

Methods and Protocols

  • Shu-Zhen Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 884)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Manipulation of Gene Expression In Vivo

    1. Front Matter
      Pages 1-1
    2. John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Bernal, Lennart Berglin, T. Michael Redmond et al.
      Pages 53-69
    3. Teri L. Belecky-Adams, Scott R. Hudson, Sarika Tiwari
      Pages 71-89
  3. Tracing Cell Fate

  4. In Vitro Systems

    1. Front Matter
      Pages 153-153
    2. Joshua Sammons, Alecia K. Gross
      Pages 167-181
    3. Run-Tao Yan, Shu-Zhen Wang
      Pages 193-199
  5. Regeneration/Stem Cells

  6. Function/Imaging

  7. Emerging Methodologies

    1. Front Matter
      Pages 287-287
    2. Laszlo Hackler Jr, Tomohiro Masuda, Verity F. Oliver, Shannath L. Merbs, Donald J. Zack
      Pages 289-304
    3. Matthew J. Brooks, Harsha Karur Rajasimha, Anand Swaroop
      Pages 319-334
    4. Rinki Ratna Priya, Harsha Karur Rajasimha, Matthew J. Brooks, Anand Swaroop
      Pages 335-351
  8. Back Matter
    Pages 363-367

About this book


In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels. These advanced methodologies have allowed and will continue to allow researchers to gain new insights into the molecular mechanisms underlying retinal development. In Retinal Development: Methods and Protocols, expert researchers in the field detail many of the protocols  used for a wide range of experiments. These include protocols  and techniques for manipulating gene expression in vivo, tracing cell fates with modernized classic blastomere manipulation in Xenopus and with Cre-based technique in mouse and in zebrafish, retinal regeneration and stem cell-based replacement, and ERG (function) recording and non-invasive imaging. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Retinal Development: Methods and Protocols provides methodologies crucial to the success of increasingly more complex and often challenging investigations in the fields of retinal development and other biological and biomedical research. 


DNA methylation ERG (function) recording Exome-seq RAN-seq chromosomal conformation capture gene inactivation generation of transgenic Xenopus in vitro systems laser microdissection miRNA non-invasive imaging retinal development therapeutic reagents

Editors and affiliations

  • Shu-Zhen Wang
    • 1
  1. 1.University of Alabama at BirminghamBirminghamUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-847-4
  • Online ISBN 978-1-61779-848-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site