Immunogenetics

Methods and Applications in Clinical Practice

  • Frank T. Christiansen
  • Brian D. Tait

Part of the Methods in Molecular Biology book series (MIMB, volume 882)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Heather Dunckley
    Pages 9-25
  3. Michael D. Varney, Alison S. L. Castley, Katri Haimila, Päivi Saavalainen
    Pages 27-46
  4. Daniel Trajanoski, Samantha J. Fidler
    Pages 47-65
  5. Linda K. Smith
    Pages 67-86
  6. Holger Nückel, Erick C. Castelli, Philippe Moreau, Crista Ochsenfarth, Peter A. Horn, Vera Rebmann
    Pages 123-142
  7. Nina Lauterbach, Christina E. M. Voorter, Marcel G. J. Tilanus
    Pages 143-158
  8. Alison S. L. Castley, O. Patricia Martinez
    Pages 159-171
  9. Yizhou Zou, Peter Stastny
    Pages 183-195
  10. Pierre-Antoine Gourraud, Jill A. Hollenbach, Thomas Barnetche, Richard M. Single, Steven J. Mack
    Pages 197-213
  11. Steven J. Mack, Pierre-Antoine Gourraud, Richard M. Single, Glenys Thomson, Jill A. Hollenbach
    Pages 215-244
  12. Jill A. Hollenbach, Steven J. Mack, Glenys Thomson, Pierre-Antoine Gourraud
    Pages 245-266
  13. Yizhou Zou, Peter Stastny
    Pages 279-288
  14. Andrea A. Zachary, Renato M. Vega, Donna P. Lucas, Mary S. Leffell
    Pages 289-308
  15. Mandvi Bharadwaj, Nicole A. Mifsud, James McCluskey
    Pages 309-337
  16. Lloyd J. D’Orsogna, Ellen M. W. van der Meer-Prins, Yvonne M. Zoet, Dave L. Roelen, Ilias I. N. Doxiadis, Frans H. J. Claas
    Pages 339-349

About this book

Introduction

The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibilty barriers to transplantation, which is the clinical paradigm of the self versus non self concept. It is now recognized that this diverse range of gene systems involved in the control of the immune response have been shown to be important in many aspects of clinical practice. As a result many new molecular and cellular methods have been developed for identifying these genes and their polymorphisms, and immunogenetic laboratories specializing in these methods have developed to support transplantation and other clinical programs. Immunogenetics: Methods and Applications in Clinical Practice focuses on methods for human clinical practice. The emphasis rests on those assays which are of established or potential clinical utility and are likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory. This volume also contains several review chapters of the MHC complex, the KIR complex, the human immunoglobulin allotypes, as well as reviews of the methods for the detection of alloreactive NK cells and the detection of HLA antibodies by solid phase assays.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Immunogenetics: Methods and Applications in Clinical Practice seeks to serve both the immunogenetics community  and the wider scientific community with a collection of detailed information and helpful tips attained by many years of experience in the field.

Keywords

DNA sequencing HLA molecules HLA typing Immunogenetic Data Major Histocompatibility Complex (HMC)

Editors and affiliations

  • Frank T. Christiansen
    • 1
  • Brian D. Tait
    • 2
  1. 1.PathWest Laboratory Medicine, Department of Clinical ImmunologyRoyal Perth HospitalPerthAustralia
  2. 2.Australian Red Cross Blood Service, National Transplant ServicesThe Royal Melbourne HospitalMelbourne VICAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-842-9
  • Copyright Information Springer Science+Business Media New York 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-841-2
  • Online ISBN 978-1-61779-842-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book