Protein Electrophoresis

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 869)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Pothur R. Srinivas
    Pages 23-28
  3. Mandy Peach, Noelle Marsh, Daniel J. MacPhee
    Pages 37-47
  4. Claudia Arndt, Stefanie Koristka, Holger Bartsch, Michael Bachmann
    Pages 49-53
  5. Engelbert Buxbaum
    Pages 55-63
  6. Syed R. Haider, Helen J. Reid, Barry L. Sharp
    Pages 81-91
  7. Marta P. Pereira Morais, John S. Fossey, Tony D. James, Jean M. H. van den Elsen
    Pages 93-109
  8. Henghang Tsai, Hon-chiu Eastwood Leung
    Pages 119-134
  9. Kim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, Peter Hoffmann
    Pages 135-146
  10. Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, Jean-Louis Viovy
    Pages 173-184
  11. Mustafa Çelebier, Clara Ibáñez, Carolina Simó, Alejandro Cifuentes
    Pages 185-195
  12. Yassel Ramos, Vladimir Besada, Lila Castellanos-Serra
    Pages 197-204
  13. Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, Jose Luis Rosa
    Pages 205-213

About this book


Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.


electrophoresis fluorescent staining gel electrophoresis polyacrylamide gels protein stains

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Oklahoma Medical Research FoundationUniv. of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.Oklahoma Medical Research FoundationOklahoma CityUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-820-7
  • Online ISBN 978-1-61779-821-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book