Plant Cell Culture Protocols

  • Víctor M. Loyola-Vargas
  • Neftalí Ochoa-Alejo

Part of the Methods in Molecular Biology book series (MIMB, volume 877)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Víctor M. Loyola-Vargas, Neftalí Ochoa-Alejo
    Pages 1-8
  3. Trevor Thorpe
    Pages 9-27
  4. Rosa M. Galáz-Ávalos, Sagrario Aguilar-Díaz, Pedro A. Xool-González, Silvia M. Huchín-May, Víctor M. Loyola-Vargas
    Pages 29-40
  5. Gregorio Godoy-Hernández, Felipe A. Vázquez-Flota
    Pages 41-48
  6. Lizbeth A. Castro-Concha, Rosa María Escobedo, María de Lourdes Miranda-Ham
    Pages 49-56
  7. Zhenfang Yin, Long Chen, Bing Zhao, Yongxing Zhu, Qiaochun Wang
    Pages 81-93
  8. Michael E. Compton
    Pages 109-127
  9. Yuri Peña-Ramírez, Juan Juárez-Gómez, José Antonio González-Rodríguez, Manuel L. Robert
    Pages 129-141
  10. Yıldız Aka Kaçar, Ben Faber
    Pages 143-151
  11. Miguel Ángel Herrera-Alamillo, Manuel L. Robert
    Pages 153-160
  12. Vanessa Prigge, Albrecht E. Melchinger
    Pages 161-172
  13. Verónica Garrocho-Villegas, María Teresa de Jesús-Olivera, Estela Sánchez Quintanar
    Pages 173-182
  14. Neftalí Ochoa-Alejo
    Pages 227-231
  15. Juntaro Kato, Masahiro Mii
    Pages 233-245
  16. José Luis Couselo, Elena Corredoira, Ana M. Vieitez, Antonio Ballester
    Pages 247-263
  17. María del Socorro Santos-Díaz, María del Carmen Barrón-Cruz
    Pages 265-270
  18. Felipe A. Vázquez-Flota, Miriam Monforte-González, Cecilia Guízar-González, Jorge Rubio-Piña, Karen Trujillo-Villanueva
    Pages 271-276
  19. Marcos Viejo, María E. Santamaría, José L. Rodríguez, Luis Valledor, Mónica Meijón, Marta Pérez et al.
    Pages 277-301
  20. Qing Liu, Yue-Qin Chen
    Pages 303-311
  21. Geovanny I. Nic-Can, Clelia De la Peña
    Pages 313-324
  22. José L. Rodríguez, Jesús Pascual, Marcos Viejo, Luis Valledor, Mónica Meijón, Rodrigo Hasbún et al.
    Pages 325-341
  23. María Tamayo-Ordoñez, Javier Huijara-Vasconselos, Adriana Quiroz-Moreno, Matilde Ortíz-García, Lorenzo Felipe Sánchez-Teyer
    Pages 343-356
  24. Cecília Tamás-Nyitrai, Huw D. Jones, László Tamás
    Pages 357-384
  25. Rubén Álvarez-Fernández, Ricardo-Javier Ordás
    Pages 385-399
  26. Anjanabha Bhattacharya, Anish Kumar, Nirali Desai, Seema Parikh
    Pages 401-406
  27. Víctor M. Loyola-Vargas
    Pages 407-417
  28. Back Matter
    Pages 427-430

About this book


Cell culture methodologies have become standard procedures in most plant laboratories. Currently,  facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.


clonal propagation culture induction epigenetic analysis haploid production organelle transformation plant biotechnology somatic embryogenesis

Editors and affiliations

  • Víctor M. Loyola-Vargas
    • 1
  • Neftalí Ochoa-Alejo
    • 2
  1. 1., Unidad de Bioquímica y Biología MoleculaCentro de Investigación Científica de YuCordemex, MéridaMexico
  2. 2., Departamento de Ingeniería Genética de PCtr Investigación, Estudios Avanzados InIrapuatoMexico

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-817-7
  • Online ISBN 978-1-61779-818-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site