Spectroscopic Methods of Analysis

Methods and Protocols

  • Wlodek M. Bujalowski

Part of the Methods in Molecular Biology book series (MIMB, volume 875)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Yi-Chun Chen, Bryan Q. Spring, Robert M. Clegg
    Pages 1-22
  3. P. Bojarski, A. Synak, L. Kułak, S. Rangelowa-Jankowska, A. Kubicki, B. Grobelna
    Pages 23-55
  4. Wieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko, Zygmunt Gryczynski, Ignacy Gryczynski
    Pages 57-65
  5. Christopher J. Fischer, Eric J. Tomko, Colin G. Wu, Timothy M. Lohman
    Pages 85-104
  6. Nicholas E. Grossoehme, David P. Giedroc
    Pages 165-192
  7. Maria Strianese, Maria Staiano, Giuseppe Ruggiero, Tullio Labella, Claudio Pellecchia, Sabato D’Auria
    Pages 193-216
  8. Ignacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko, Pabak Sarkar, Julian Borejdo et al.
    Pages 217-229
  9. Mandy Siu Yu Lung, Paul Pilowsky, Ewa M. Goldys
    Pages 241-251
  10. Philip Butterworth, Henrique T. M. C. M. Baltar, Martin Kratzmeier, Ewa M. Goldys
    Pages 253-262
  11. Wieslaw I. Gruszecki, Rafal Luchowski, Wojciech Grudzinski, Zygmunt Gryczynski, Ignacy Gryczynski
    Pages 263-269
  12. Goran Pljevaljčić, Rae Robertson-Anderson, Edwin van der Schans, David Millar
    Pages 271-295
  13. Liang Ma, Meixiang Xu, Andres F. Oberhauser
    Pages 297-310
  14. Julian Borejdo, Danuta Szczesna-Cordary, Priya Muthu, Prasad Metticolla, Rafal Luchowski, Zygmunt Gryczynski et al.
    Pages 311-334
  15. Back Matter
    Pages 393-397

About this book


Quantitative elucidation of structural, energetic and dynamic aspects of macromolecular interactions is indispensable for understanding the functional activities of biomolecules and their interactions. The optical spectroscopic methods are not confined to small molecules or macromolecules but permit the studies of even the largest biological systems in their full splendor, including the living cell. In, Spectroscopic Methods of Analysis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study properties of individual macromolecules, their complexes, organelles, and cells, using optical spectroscopic techniques. These include methods and approaches for experimental and theoretical analyses of fluorescence properties of the examined systems, single molecule approaches, electronic absorption, and electro-optical analyses of macromolecular interactions, structures, and dynamics. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Spectroscopic Methods of Analysis :  Methods and Protocols seeks to aid scientists in the further study of optical spectroscopic methods.


RNA folding Single molecule biological systems birefringence electric dichroism electro-optical analysis fluorescence properties macromolecules ribonucleoprotein assembly spectroscopic methods

Editors and affiliations

  • Wlodek M. Bujalowski
    • 1
  1. 1.Dept. Biochemistry & Molecular BiologyUniversity of Texas Medical BranchGalvestonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-805-4
  • Online ISBN 978-1-61779-806-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site