Methods and Protocols

  • Alice Pébay
  • Kursad Turksen

Part of the Methods in Molecular Biology book series (MIMB, volume 874)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Melissa R. Pitman, Duyen H. Pham, Stuart M. Pitson
    Pages 21-31
  3. Matt D. Medlin, Joan M. Taylor, Christopher P. Mack
    Pages 89-97
  4. Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta, Masaru Ishii
    Pages 129-139
  5. Elisabetta Meacci, Francesca Bini, Chiara Battistini
    Pages 155-165
  6. Phillip Callihan, Shelley B. Hooks
    Pages 193-200
  7. Shih-Chi Su, Kayla J. Bayless
    Pages 201-213
  8. Back Matter
    Pages 215-221

About this book


Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  In Sphingosine-1-Phosphate: Methods and Protocols, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology.  As a volume in the Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.


Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols is a key resource for scientists working in this expanding and dynamic field.


Lysophospholipids Physiopathology S1P S1P Signalling Sphingosine-1-phosphate

Editors and affiliations

  • Alice Pébay
    • 1
  • Kursad Turksen
    • 2
  1. 1.University of MelbourneParkvilleAustralia
  2. 2.Sprott Centre for Stem Cell Research, Regenerative Medicine ProgramOttawa Hospital Research InstituteOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-799-6
  • Online ISBN 978-1-61779-800-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site