Human Embryonic Stem Cells Handbook

  • Kursad Turksen

Part of the Methods in Molecular Biology book series (MIMB, volume 873)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ana Maria Fraga, Érica Sara Souza de Araújo, Raquel Stabellini, Naja Vergani, Lygia V. Pereira
    Pages 1-12
  3. Amparo Galán, Carlos Simón
    Pages 13-32
  4. Mikael C. O. Englund, Catharina Ellerström, Katarina Andersson, Karin Noaksson, Johan Hyllner
    Pages 53-68
  5. Guoliang Meng, Derrick E. Rancourt
    Pages 69-80
  6. Martin Muñoz-Lopez, Marta Garcia-Cañadas, Angela Macia, Santiago Morell, Jose L. Garcia-Perez
    Pages 113-125
  7. Ronak Shetty, Maneesha S. Inamdar
    Pages 151-161
  8. Ahmi Ben-Yehudah, Mira Malcov, Tsvia Frumkin, Dalit Ben-Yosef
    Pages 179-207
  9. Hossein Baharvand, Mehran Rezaei Larijani, Maryam Yousefi
    Pages 217-226
  10. Aaron M. Elliott, Kristi A. Hohenstein Elliott, Anja Kammesheidt
    Pages 261-267
  11. Linda Harkness, Tatyana A. Prokhorova, Moustapha Kassem, Blagoy Blagoev
    Pages 297-305
  12. Back Matter
    Pages 307-313

About this book


Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult.  In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology.  Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.


HESC Human embryonic stem cells Regenerative medicine Restorative properties Stem cell characterization Stem cell isolation

Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Sprott Centre for Stem Cell Research, Regenerative Medicine ProgramOttawa Hospital Research InstituteOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-793-4
  • Online ISBN 978-1-61779-794-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site