Recombinant Protein Production in Yeast

Methods and Protocols

  • Roslyn M. Bill

Part of the Methods in Molecular Biology book series (MIMB, volume 866)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Richard A. J. Darby, Stephanie P. Cartwright, Marvin V. Dilworth, Roslyn M. Bill
    Pages 11-23
  3. Christel Logez, Fatima Alkhalfioui, Bernadette Byrne, Renaud Wagner
    Pages 25-40
  4. Mohammed Jamshad, Richard A. J. Darby
    Pages 57-63
  5. Shweta Singh, Adrien Gras, Cedric Fiez-Vandal, Magdalena Martinez, Renaud Wagner, Bernadette Byrne
    Pages 65-73
  6. Sarah J. Routledge, Roslyn M. Bill
    Pages 87-97
  7. Sarah J. Routledge, Michelle Clare
    Pages 99-113
  8. Nagamani Bora, Zharain Bawa, Roslyn M. Bill, Martin D. B. Wilks
    Pages 115-127
  9. Shane M. Palmer, Edmund R. S. Kunji
    Pages 129-155
  10. Shane M. Palmer, Edmund R. S. Kunji
    Pages 165-179
  11. Zharain Bawa, Richard A. J. Darby
    Pages 181-190
  12. David Drew, Hyun Kim
    Pages 191-195
  13. Shweta Singh, Adrien Gras, Cedric Fiez-Vandal, Magdalena Martinez, Renaud Wagner, Bernadette Byrne
    Pages 197-207

About this book

Introduction

In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.

Keywords

bioreactor formats enzymes expression vectors growth variables high-yielding clones medium composition pharmaceutical proteins protein behavior recombinant proteins yeast

Editors and affiliations

  • Roslyn M. Bill
    • 1
  1. 1.School of Life and Health SciencesAston UniversityBirminghamUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-770-5
  • Copyright Information Springer Science+business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-769-9
  • Online ISBN 978-1-61779-770-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book