Influenza Virus

Methods and Protocols

  • Yoshihiro Kawaoka
  • Gabriele Neumann

Part of the Methods in Molecular Biology book series (MIMB, volume 865)

Table of contents

  1. Front Matter
    Pages i-x
  2. Yoshihiro Kawaoka, Gabriele Neumann
    Pages 1-9
  3. Scott Krauss, David Walker, Robert G. Webster
    Pages 11-24
  4. Alexander Klimov, Amanda Balish, Vic Veguilla, Hong Sun, Jarad Schiffer, Xiuhua Lu et al.
    Pages 25-51
  5. Kirsten St. George
    Pages 53-69
  6. Mikhail N. Matrosovich, Alexandra S. Gambaryan
    Pages 71-94
  7. Edwin J. B. Veldhuis Kroeze, Thijs Kuiken, Albert D. M. E. Osterhaus
    Pages 127-146
  8. Klaus Stöhr, Doris Bucher, Tony Colgate, John Wood
    Pages 147-162
  9. Hong Jin, Zhongying Chen, Jonathan Liu, George Kemble
    Pages 163-174
  10. Bin Zhou, David E. Wentworth
    Pages 175-192
  11. Gabriele Neumann, Makoto Ozawa, Yoshihiro Kawaoka
    Pages 193-206
  12. Gavin J. D. Smith, Justin Bahl, Dhanasekaran Vijaykrishna
    Pages 207-227
  13. Back Matter
    Pages 229-234

About this book


Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals – ion channel and neuraminidase inhibitors. Recently, a method of the artificial generation of an influenza virus was established. This system has been instrumental in the development of novel influenza vaccines and in the understanding of viral pathogenicity and the functions of viral proteins. Influenza Virus: Methods and Protocols summarizes the current techniques that have made this progress possible, ranging from protocols for virus isolation, growth, and subtyping to procedures for the efficient generation of any influenza virus. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Influenza Virus: Methods and Protocols seeks to serve both professionals and novices with the techniques used in numerous laboratories around the world that are, thus, the building blocks that underpin almost all influenza virus research.


antigenic characterization molecular virology virus isolation virus subtyping virus titration

Editors and affiliations

  • Yoshihiro Kawaoka
    • 1
  • Gabriele Neumann
    • 2
  1. 1.School of Veterinary Medicine, Dept. Pathobiological ScienceUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Influenza Research Institute (IRI), Dept. Pathobiological ScienceUniversity of Wisconsin-MadisonMadisonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-620-3
  • Online ISBN 978-1-61779-621-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site