Difference Gel Electrophoresis (DIGE)

Methods and Protocols

  • Rainer Cramer
  • Reiner Westermeier

Part of the Methods in Molecular Biology book series (MIMB, volume 854)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Fundamentals

    1. Front Matter
      Pages 1-1
    2. Jonathan S. Minden
      Pages 3-8
    3. Phil Beckett
      Pages 9-19
    4. Ingo Vormbrock, Sonja Hartwig, Stefan Lehr
      Pages 21-30
    5. Witold M. Winnik, Robert M. DeKroon, Joseph S. Y. Jeong, Mihaela Mocanu, Jennifer B. Robinette, Cristina Osorio et al.
      Pages 47-66
    6. Michael E. Jung, Wan-Joong Kim, Nuraly K. Avliyakulov, Merve Oztug, Michael J. Haykinson
      Pages 67-85
  3. Methods

    1. Front Matter
      Pages 87-87
    2. Georg J. Arnold, Thomas Fröhlich
      Pages 89-112
    3. Hong-Lin Chan, John Sinclair, John F. Timms
      Pages 113-128
    4. Robert M. DeKroon, Jennifer B. Robinette, Cristina Osorio, Joseph S. Y. Jeong, Eric Hamlett, Mihaela Mocanu et al.
      Pages 129-143
    5. Katrin Peters, Hans-Peter Braun
      Pages 145-154
    6. Cecilia Gelfi, Sara De Palma
      Pages 155-168
    7. Cynthia Liang, Gek San Tan, Maxey C. M. Chung
      Pages 181-194
    8. Alexandra Graf, Rudolf Oehler
      Pages 195-206
    9. Megan Penno, Matthias Ernst, Peter Hoffmann
      Pages 207-220
  4. Applications in Clinical Proteomics

    1. Front Matter
      Pages 221-221
    2. Keun Na, Min-Jung Lee, Hye-Jin Jeong, Hoguen Kim, Young-Ki Paik
      Pages 223-237

About this book

Introduction

Protein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavily on the past and future advances of protein purification and analysis methods.  DIGE, being able to quantify proteins in their intact form, is one of a few methods that can facilitate this type of analysis and still provide the protein isoforms in an MS-compatible state for further identification and characterization with high analytical sensitivity. Differential Gel Electrophoresis: Methods and Protocols introduces the concept of DIGE and its advantages in quantitative protein analysis. It provides detailed protocols and important notes on the practical aspects of DIGE with both generic and specific applications in the various areas of Quantitative Proteomics. Divided into four concise sections, this detailed volume opens with the basics of DIGE, the technique and its practical details with a focus on the planning of a DIGE experiment and its data analysis. The next section introduces various DIGE methods from those employed by scientists world-wide to more novel methods, providing a glance at what is on the horizon in the DIGE world.  The volume closes with an overview of the wide range of DIGE applications from Clinical Proteomics to Animal, Plant, and Microbial Proteomics applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Differential Gel Electrophoresis: Methods and Protocols can be used by novices with some background in biochemistry or molecular biology as well as by experts in Proteomics who would like to deepen their understanding of DIGE and its employment in many hyphenations and application areas.  With its many protocols, applications, and methodological variants, it is also a unique reference for all who seek fundamental details on the working principle of DIGE and ideas for possible future uses of DIGE in novel analytical approaches.

Keywords

2D DIGE biomarker discovery mass spectrometry proteomic profiling quantitative proteomics

Editors and affiliations

  • Rainer Cramer
    • 1
  • Reiner Westermeier
    • 2
  1. 1.BioCentreUniversity of ReadingReadingUnited Kingdom
  2. 2.SERVA Electrophoresis GmbHHeidelbergGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-573-2
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-572-5
  • Online ISBN 978-1-61779-573-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book