Gene Synthesis

Methods and Protocols

  • Jean Peccoud

Part of the Methods in Molecular Biology book series (MIMB, volume 852)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Assembly of Oligonucleotides in Synthons

    1. Front Matter
      Pages 1-1
    2. Julie A. Marchand, Jean Peccoud
      Pages 3-10
    3. Mo Chao Huang, Wai Chye Cheong, Hongye Ye, Mo-Huang Li
      Pages 23-34
    4. Tuval Ben Yehezkel, Gregory Linshiz, Ehud Shapiro
      Pages 35-47
  3. Synthon Assembly

    1. Front Matter
      Pages 49-49
    2. Mamie Z. Li, Stephen J. Elledge
      Pages 51-59
    3. Olivia Ho-Shing, Kin H. Lau, William Vernon, Todd T. Eckdahl, A. Malcolm Campbell
      Pages 61-76
    4. Narayana Annaluru, Héloïse Muller, Sivaprakash Ramalingam, Karthikeyan Kandavelou, Viktoriya London, Sarah M. Richardson et al.
      Pages 77-95
    5. Kamonchai Cha-aim, Hisashi Hoshida, Tomoaki Fukunaga, Rinji Akada
      Pages 97-110
    6. Héloïse Muller, Narayana Annaluru, Joy Wu Schwerzmann, Sarah M. Richardson, Jessica S. Dymond, Eric M. Cooper et al.
      Pages 133-150
    7. Gregory Linshiz, Tuval Ben Yehezkel, Ehud Shapiro
      Pages 151-163
    8. Gwynedd A. Benders
      Pages 165-180
    9. Jeronimo Cello, Steffen Mueller
      Pages 181-193
  4. Software for Gene Synthesis

    1. Front Matter
      Pages 195-195
    2. Alan Villalobos, Mark Welch, Jeremy Minshull
      Pages 197-213
    3. Mo-Huang Li, Marcus Bode, Mo Chao Huang, Wai Chye Cheong, Li Shi Lim
      Pages 225-234
    4. Sarah M. Richardson, Steffi Liu, Jef D. Boeke, Joel S. Bader
      Pages 235-247
  5. Education and Security

    1. Front Matter
      Pages 249-249
    2. Wayne Materi
      Pages 251-272
    3. Eric M. Cooper, Helöise Müller, Srinivasan Chandrasegaran, Joel S. Bader, Jef D. Boeke
      Pages 273-283
    4. Ali Nouri, Christopher F. Chyba
      Pages 285-296
  6. Back Matter
    Pages 297-297

About this book


The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.


Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.


DNA PCR-based methods ad hoc methods bacterial genomes biosecurity impacts cloning techniques de novo fabrication gene synthesis oligonucleotides synthons

Editors and affiliations

  • Jean Peccoud
    • 1
  1. 1.Virginia Bioinformatics InstituteVirginia TechBlacksburgUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Sceince+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-563-3
  • Online ISBN 978-1-61779-564-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site