Table of contents

  1. Front Matter
    Pages i-xii
  2. Tara L. Fulton
    Pages 1-11
  3. Tara L. Fulton, Stephen M. Wagner, Beth Shapiro
    Pages 29-35
  4. Melanie Kuch, Hendrik Poinar
    Pages 37-42
  5. Paula F. Campos, Thomas M. P. Gilbert
    Pages 43-49
  6. Andrew A. Clack, Ross D. E. MacPhee, Hendrik N. Poinar
    Pages 51-56
  7. Charlotte L. Oskam, Michael Bunce
    Pages 65-70
  8. Logan Kistler
    Pages 71-79
  9. Paula F. Campos, Thomas M. P. Gilbert
    Pages 81-85
  10. Tara L. Fulton, Mathias Stiller
    Pages 111-119
  11. Michael Bunce, Charlotte L. Oskam, Morten E. Allentoft
    Pages 121-132
  12. Mathias Stiller, Tara L. Fulton
    Pages 133-141
  13. Adrian W. Briggs, Patricia Heyn
    Pages 143-154
  14. Michael Knapp, Mathias Stiller, Matthias Meyer
    Pages 155-170

About this book

Introduction

Research into ancient DNA began more than 25 years ago with the publication of short mitochondrial DNA sequence fragments from the quagga, an extinct relative of the zebra. Ancient DNA research really gained momentum following the invention of PCR, which allowed millions of copies to be made of the few remaining DNA molecules preserved in fossils and museum specimens.  In Ancient DNA: Methods and Protocols expert researchers in the field describe many of the protocols that are now commonly used to study ancient DNA. These include instructions for setting up an ancient DNA laboratory, extraction protocols for a wide range of different substrates, details of laboratory techniques including PCR and NGS library preparation, and suggestions for appropriate analytical approaches to make sense of the sequences obtained. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Ancient DNA: Methods and Protocols seeks to aid scientists in the further study of ancient DNA and the methodological approaches in ancient research.

Keywords

NGS PCR Pleistocene-age DNA ancient DNA ancient bone bone hydroxyl-apatite hydroxyl-apatite crystals mitochondrial genomes phylogeographic patterns soft tissue

Editors and affiliations

  • Beth Shapiro
    • 1
  • Michael Hofreiter
    • 2
  1. 1.Dept. of Ecology and Evolutionary BiologUniversity of California Santa CruzSanta CruzUSA
  2. 2., Department of Biology (Area 2)University of YorkYorkUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-516-9
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-515-2
  • Online ISBN 978-1-61779-516-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book