Plant Fungal Pathogens

Methods and Protocols

  • Melvin D. Bolton
  • Bart P.H.J. Thomma

Part of the Methods in Molecular Biology book series (MIMB, volume 835)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Andriy Kovalchuk, Stefan S. Weber, Jeroen G. Nijland, Roel A. L. Bovenberg, Arnold J. M. Driessen
    Pages 1-16
  3. Rasmus John Normand Frandsen, Mette Frandsen, Henriette Giese
    Pages 17-45
  4. Farnusch Kaschani, Christian Gu, Renier A. L. van der Hoorn
    Pages 47-59
  5. Delphine Vincent, Kar-Chun Tan, Liam Cassidy, Peter S. Solomon, Richard P. Oliver
    Pages 75-96
  6. Steven J. Klosterman
    Pages 121-132
  7. Elizabeth Adams, Danielle Emerson, Sean Croker, Hye-Seon Kim, Shannon Modla, Seogchan Kang et al.
    Pages 151-164
  8. Joel P. A. Gummer, Christian Krill, Lauren Du Fall, Ormonde D. C. Waters, Robert D. Trengove, Richard P. Oliver et al.
    Pages 237-254
  9. Sucheta Tripathy, Rays H. Y. Jiang
    Pages 271-294
  10. Adrienne R. Hardham
    Pages 295-309
  11. Benjamin M. Nitsche, Arthur F. J. Ram, Vera Meyer
    Pages 311-331
  12. Zahi K. Atallah, Krishna V. Subbarao
    Pages 333-363
  13. Gary A. Secor, Viviana V. Rivera
    Pages 385-392
  14. Shiv D. Kale, Brett M. Tyler
    Pages 393-414
  15. H. Charlotte van der Does, Martijn Rep
    Pages 427-437
  16. Rohan Lowe, Mélanie Jubault, Gail Canning, Martin Urban, Kim E. Hammond-Kosack
    Pages 439-455
  17. J. B. Ridenour, R. L. Hirsch, B. H. Bluhm
    Pages 457-479
  18. B. Lievens, A. Justé, K. A. Willems
    Pages 491-507
  19. Eva H. Stukenbrock, Julien Y. Dutheil
    Pages 531-548
  20. Tara L. Rintoul, Quinn A. Eggertson, C. André Lévesque
    Pages 549-569
  21. Rahim Mehrabi, Sarrah Ben M’Barek, Abbas Saidi, Masoud Abrinbana, Pierre J. G. M. de Wit, Gert H. J. Kema
    Pages 571-583
  22. Rahim Mehrabi, Masatoki Taga, Mostafa Aghaee, Pierre J. G. M. de Wit, Gert H. J. Kema
    Pages 591-602
  23. Javier A. Delgado, Steven Meinhardt, Samuel G. Markell, Rubella S. Goswami
    Pages 611-622
  24. Shaobin Zhong, Yueqiang Leng, Melvin D. Bolton
    Pages 623-633
  25. Back Matter
    Pages 645-648

About this book


Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor.  In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Plant Fungal Pathogens: Methods and Protocols seeks to aid scientists in the further study in current techniques that cover a wide-range of methods to study molecular aspects of pathogenesis.


Plant Fungal Pathogens effectors fungal biology fungal genome sequencing fungal transformation pathosystem

Editors and affiliations

  • Melvin D. Bolton
    • 1
  • Bart P.H.J. Thomma
    • 2
  1. 1.Northern Crops Science LaboratoryUSDA-ARSFargoUSA
  2. 2.Lab. PhytopathologyWageningen UniversityWageningenNetherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-500-8
  • Online ISBN 978-1-61779-501-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site