Two Hybrid Technologies

Methods and Protocols

  • Bernhard Suter
  • Erich E. Wanker

Part of the Methods in Molecular Biology book series (MIMB, volume 812)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Roman Häuser, Thorsten Stellberger, Seesandra V. Rajagopala, Peter Uetz
    Pages 1-20
  3. Roman Häuser, Thorsten Stellberger, Seesandra V. Rajagopala, Peter Uetz
    Pages 21-38
  4. George G. Roberts III, Jodi R. Parrish, Bernardo A. Mangiola, Russell L. Finley Jr.
    Pages 39-61
  5. Josephine M. Worseck, Arndt Grossmann, Mareike Weimann, Anna Hegele, Ulrich Stelzl
    Pages 63-87
  6. Lionel Tafforeau, Chantal Rabourdin-Combe, Vincent Lotteau
    Pages 103-120
  7. Xiaofeng Xin, Charles Boone, Nicolas Thierry-Mieg
    Pages 147-159
  8. Jingkai Yu, Thilakam Murali, Russell L. Finley Jr.
    Pages 161-174
  9. Gautam Chaurasia, Matthias Futschik
    Pages 175-188
  10. John S. Reece-Hoyes, Albertha J. M. Walhout
    Pages 189-208
  11. Julia Petschnigg, Victoria Wong, Jamie Snider, Igor Stagljar
    Pages 225-244
  12. Mandana Rezwan, Nicolas Lentze, Lukas Baumann, Daniel Auerbach
    Pages 245-258
  13. Kathryn Moncivais, Zhiwen Jonathan Zhang
    Pages 259-273
  14. Sam Lievens, Nele Vanderroost, Dieter Defever, José Van der Heyden, Jan Tavernier
    Pages 283-294
  15. Bertrand Van Schoubroeck, Koen Van Acker, Géry Dams, Dirk Jochmans, Reginald Clayton, Jan Martin Berke et al.
    Pages 295-307
  16. Back Matter
    Pages 325-329

About this book


Protein–protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols  supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.


interactomics protein-protein interaction detection methods virus-human cell interactomes yeast two-hybrid approach yeast two-hybrid high-throughput screens

Editors and affiliations

  • Bernhard Suter
    • 1
  • Erich E. Wanker
    • 2
  1. 1.Molekulare Medizin (MDC)Max-Delbrück-Centrum fürBerlin-BuchGermany
  2. 2.Max-Delbrück-Centrum-Für Molekulare MediBerlinGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-454-4
  • Online ISBN 978-1-61779-455-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site