Amino Acid Analysis

Methods and Protocols

  • Michail A. Alterman
  • Peter Hunziker

Part of the Methods in Molecular Biology book series (MIMB, volume 828)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Akiyoshi Hirayama, Tomoyoshi Soga
    Pages 77-82
  3. Olga A. Mirgorodskaya, Roman Körner, Yuri P. Kozmin, Peter Roepstorff
    Pages 115-120
  4. Katja Dettmer, Axel P. Stevens, Stephan R. Fagerer, Hannelore Kaspar, Peter J. Oefner
    Pages 165-181
  5. Giuseppe Giordano, Iole Maria Di Gangi, Antonina Gucciardi, Mauro Naturale
    Pages 219-242
  6. Dimitrios Tsikas, Anja Mitschke, Frank-Mathias Gutzki
    Pages 255-270
  7. Tobias Langrock, Ralf Hoffmann
    Pages 271-280
  8. Michelle L. Colgrave, Peter G. Allingham, Kerri Tyrrell, Alun Jones
    Pages 291-303
  9. Björn Thiele, Nadine Stein, Marco Oldiges, Diana Hofmann
    Pages 317-328
  10. Ine Rombouts, Bert Lagrain, Lieve Lamberts, Inge Celus, Kristof Brijs, Jan A. Delcour
    Pages 329-337
  11. Jennifer A. Tripp, James S. O. McCullagh
    Pages 339-350
  12. Back Matter
    Pages 359-363

About this book


Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology.  Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.


capillary electrophoresis chromatography tandem mass spectrometry underivatized amino acids unusual amino acids

Editors and affiliations

  • Michail A. Alterman
    • 1
  • Peter Hunziker
    • 2
  1. 1.Ctr. Biologics Evaluation & Research, Div. Cellular & Gene TherapiesU.S. Food and Drug AdministrationBethesdaUSA
  2. 2.Functional Genomics Center Zürich, Protein Analysis GroupUniversität ZürichZürichSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-444-5
  • Online ISBN 978-1-61779-445-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site