Book Volume 828 2012

Amino Acid Analysis

Methods and Protocols


ISBN: 978-1-61779-444-5 (Print) 978-1-61779-445-2 (Online)

Table of contents (28 protocols)

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  1. Front Matter

    Pages i-xiv

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    Pages 1-11

    Rapid LC–MS/MS Profiling of Protein Amino Acids and Metabolically Related Compounds for Large-Scale Assessment of Metabolic Phenotypes

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    Pages 13-28

    Combination of an AccQ·Tag-Ultra Performance Liquid Chromatographic Method with Tandem Mass Spectrometry for the Analysis of Amino Acids

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    Pages 29-38

    Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis

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    Pages 39-46

    Analysis of Underivatized Amino Acids: Zwitterionic Hydrophilic Interaction Chromatography Combined with Triple Quadrupole Tandem Mass Spectrometry

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    Pages 47-54

    Amino Acid Analysis via LC–MS Method After Derivatization with Quaternary Phosphonium

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    Pages 55-62

    Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass Spectrometry

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    Pages 63-75

    A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids

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    Pages 77-82

    Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry

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    Pages 83-99

    New Advances in Amino Acid Profiling by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry

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    Pages 101-114

    Optimal Conditions for the Direct RP-HPLC Determination of Underivatized Amino Acids with Online Multiple Detection

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    Pages 115-120

    Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry

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    Pages 121-135

    Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass Spectrometry

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    Pages 137-152

    Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Chiral and Nonchiral Amino Acid Analysis by Gas Chromatography

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    Pages 153-164

    The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determination of Plasma Tryptophan and Other Amino Acids

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    Pages 165-181

    Amino Acid Analysis in Physiological Samples by GC–MS with Propyl Chloroformate Derivatization and iTRAQ–LC–MS/MS

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    Pages 183-200

    Automated Analysis of Primary Amino Acids in Plasma by High-Performance Liquid Chromatography

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    Pages 201-217

    RP-LC of Phenylthiocarbamyl Amino Acid Adducts in Plasma Acetonitrile Extracts: Use of Multiple Internal Standards and Variable Wavelength UV Detection

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    Pages 219-242

    Quantification of Underivatised Amino Acids on Dry Blood Spot, Plasma, and Urine by HPLC–ESI–MS/MS

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    Pages 243-254

    Capillary Electrophoresis of Free Amino Acids in Physiological Fluids Without Derivatization Employing Direct or Indirect Absorbance Detection

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    Pages 255-270

    Measurement of 3-Nitro-Tyrosine in Human Plasma and Urine by Gas Chromatography-Tandem Mass Spectrometry

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