Germline Development

Methods and Protocols

  • Wai-Yee Chan
  • Le Ann Blomberg

Part of the Methods in Molecular Biology book series (MIMB, volume 825)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Male Germline

    1. Front Matter
      Pages 1-1
    2. Zuping He, Maria Kokkinaki, Jiji Jiang, Wenxian Zeng, Ina Dobrinski, Martin Dym
      Pages 45-57
    3. Catherine Boucheron, Vanessa Baxendale
      Pages 59-66
    4. Tin-Lap Lee, Amy Xiao, Owen M. Rennert
      Pages 105-114
    5. Hoi-Hung Cheung, Tin-Lap Lee, Owen M. Rennert, Wai-Yee Chan
      Pages 115-126
    6. Alexander I. Agoulnik, Zaohua Huang, Lydia Ferguson
      Pages 127-147
  3. Female Germline

  4. Back Matter
    Pages 253-256

About this book


The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In Germline Development: Methods and Protocols, expert researchers provide a wide-ranging collection of techniques that can be applied to the study of both the male and female germline.  As the techniques used for isolating and studying male and female germ cells are slightly dissimilar, the different technologies are grouped under two sections in this volume, namely, male germline and female germline.  This collection brings the field of germline developmental studies together such that readers can find, in a single volume, techniques that allow the isolation of gonads with germ cells at different stages of development, the study of the genomics and epigenomics of germ cells, and an examination of the role of single genes in the developmental potential and function of germ cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and the avoidance of known pitfalls. 


Comprehensive and cutting-edge, Germline Development: Methods and Protocols serves as a comprehensive text to individuals, particularly researchers in reproductive and developmental biology, who are interested in studying cells of the germ lineage.


Germ cells Germinal stem cells Germline development Male and the female germline Regenerative studies Sperm or oocyte

Editors and affiliations

  • Wai-Yee Chan
    • 1
  • Le Ann Blomberg
    • 2
  1. 1., School of Biomedical SciencesThe Chinese University of Hong KongShatin, New TerritoriesHong Kong/PR China
  2. 2., Animal BiosciencesARS-USDABeltsvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-435-3
  • Online ISBN 978-1-61779-436-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site