Next-Generation MicroRNA Expression Profiling Technology

Methods and Protocols

  • Jian-Bing Fan

Part of the Methods in Molecular Biology book series (MIMB, volume 822)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Willemijn M. Gommans, Eugene Berezikov
    Pages 1-18
  3. James Hurley, Doug Roberts, Andrew Bond, David Keys, Caifu Chen
    Pages 33-52
  4. Rui Shi, Ying-Husan Sun, Xing-Hai Zhang, Vincent L. Chiang
    Pages 53-66
  5. Boye Schnack Nielsen
    Pages 67-84
  6. Petula N. D’Andrade, Stephanie Fulmer-Smentek
    Pages 85-102
  7. Jing Chen, Craig S. April, Jian-Bing Fan
    Pages 103-116
  8. Suzanne Dee, Robert C. Getts
    Pages 117-129
  9. Markus Beier, Valesca Boisguérin
    Pages 143-152
  10. Xiaochuan Zhou, Qi Zhu, Christoph Eicken, Nijing Sheng, Xiaolin Zhang, Litao Yang et al.
    Pages 153-182
  11. Ana Raquel Soares, Patrícia M. Pereira, Manuel A. S. Santos
    Pages 189-204
  12. Philipp Kapranov, Fatih Ozsolak, Patrice M. Milos
    Pages 219-232
  13. Andreas Ruepp, Andreas Kowarsch, Fabian Theis
    Pages 249-260
  14. Barbara D’haene, Pieter Mestdagh, Jan Hellemans, Jo Vandesompele
    Pages 261-272
  15. Preethi H. Gunaratne, Cristian Coarfa, Benjamin Soibam, Arpit Tandon
    Pages 273-288
  16. William Ritchie, John E. J. Rasko
    Pages 289-293
  17. Xiaomin Zhong, George Coukos, Lin Zhang
    Pages 295-306
  18. Svenja Debey-Pascher, Jing Chen, Thorsten Voss, Andrea Staratschek-Jox
    Pages 307-338
  19. Back Matter
    Pages 339-341

About this book


The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years.  In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today.  This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development.  Written in the highly successful Methods in Molecular Biology™ series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.


Databases MicroRNA expression profiling Next-generation technology RT-PCR methods Sequencing technologies miRNA

Editors and affiliations

  • Jian-Bing Fan
    • 1
  1. 1.Illumina, Inc.San DiegoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-426-1
  • Online ISBN 978-1-61779-427-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site