Human Cell Culture Protocols

  • Ragai R. Mitry
  • Robin D. Hughes

Part of the Methods in Molecular Biology book series (MIMB, volume 806)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Christina Philippeos, Robin D. Hughes, Anil Dhawan, Ragai R. Mitry
    Pages 1-13
  3. Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, Hanno Huwer, Claus-Michael Lehr
    Pages 31-42
  4. Peyman Björklund, Per Hellman
    Pages 43-53
  5. Shanta J. Persaud, Bo Liu, Peter M. Jones
    Pages 55-71
  6. María José Gómez-Lechón, Agustín Lahoz, José V. Castell, María Teresa Donato
    Pages 87-97
  7. Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, Peter Büchler, Michael Schoenberg, Jörg Kleeff et al.
    Pages 99-120
  8. Martina Zimmermann, Timo Weiland, Michael Bitzer, Ulrich M. Lauer
    Pages 121-136
  9. Pierre Chailler, Jean-François Beaulieu, Daniel Ménard
    Pages 137-155
  10. Heather M. Wilson, Keith N. Stewart
    Pages 187-201
  11. Kirstin A. Carswell, Mi-Jeong Lee, Susan K. Fried
    Pages 203-214
  12. Diane Proudfoot, Catherine Shanahan
    Pages 251-263
  13. Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, Dimitrios P. Bogdanos
    Pages 275-285
  14. Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani, Yun Ma
    Pages 287-299
  15. Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, Ko Hashimoto, Darren A. Plumb et al.
    Pages 301-336
  16. Alison Gartland, Robin M. H. Rumney, Jane P. Dillon, James A. Gallagher
    Pages 337-355
  17. Ankita Agrawal, James A. Gallagher, Alison Gartland
    Pages 357-375
  18. Moustafa I. Hafez, Anne Sandison, Richard R. H. Coombs, Ian D. McCarthy, Al-Shymaa M. Hafez
    Pages 377-383
  19. Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, Alberto Quaglia
    Pages 385-392
  20. Robert Thomas, Elizabeth Ratcliffe
    Pages 393-406
  21. Back Matter
    Pages 427-435

About this book


Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.  This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues.  With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.


Biocompatible scaffolds Cell function Diseased tissues Hepatocytes Human stem cells Intracellular mechanisms Laser microdissection Pancreatic cells

Editors and affiliations

  • Ragai R. Mitry
    • 1
  • Robin D. Hughes
    • 2
  1. 1.Institute of Liver StudiesKing’s College HospitalLondonUnited Kingdom
  2. 2.School of Medicine, Inst. Liver StudiesKing's College LondonLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-366-0
  • Online ISBN 978-1-61779-367-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site