Unnatural Amino Acids

Methods and Protocols

  • Loredano Pollegioni
  • Stefano Servi

Part of the Methods in Molecular Biology book series (MIMB, volume 794)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Synthesis of Unnatural Amino Acids

    1. Front Matter
      Pages 1-1
    2. László Poppe, Csaba Paizs, Klaudia Kovács, Florin-Dan Irimie, Beáta Vértessy
      Pages 3-19
    3. Paola D’Arrigo, Davide Tessaro
      Pages 21-35
    4. Gwynneth F. Matcher, Rosemary A. Dorrington, Stephanie G. Burton
      Pages 37-54
    5. Thierry Gefflaut, Zeinab Assaf, Martine Sancelme
      Pages 55-72
    6. Pere Clapés, Jesús Joglar, Mariana Gutiérrez
      Pages 73-85
    7. Francisco Javier Las Heras-Vázquez, Josefa María Clemente-Jiménez, Sergio Martínez-Rodríguez, Felipe Rodríguez-Vico
      Pages 87-104
  3. Application of Unnatural Amino Acids

  4. Use of Unnatural Amino Acids in Protein Synthesis

    1. Front Matter
      Pages 185-185
    2. Nobumasa Hino, Kensaku Sakamoto, Shigeyuki Yokoyama
      Pages 215-228
    3. Paul Majkut, Verena Böhrsch, Remigiusz Serwa, Michael Gerrits, Christian P. R. Hackenberger
      Pages 241-249
  5. d-Amino Acids: Analysis and Applications

    1. Front Matter
      Pages 251-251
    2. George H. Fisher, Mara Tsesarskaia
      Pages 253-264
    3. Gianluca Molla, Luciano Piubelli, Federica Volontè, Mirella S. Pilone
      Pages 273-289
    4. Silvia Sacchi, Elena Rosini, Laura Caldinelli, Loredano Pollegioni
      Pages 313-324
    5. Noriko Fujii, Norihiko Fujii
      Pages 325-335
  6. Enzymes Active on d-Amino Acids

    1. Front Matter
      Pages 355-355
    2. Florian Baumgart, Clara Aicart-Ramos, Ignacio Rodriguez-Crespo
      Pages 357-366
    3. Masumi Katane, Masae Sekine, Hiroshi Homma
      Pages 367-379
    4. Gabriella Tedeschi, Loredano Pollegioni, Armando Negri
      Pages 381-395
  7. Back Matter
    Pages 407-409

About this book


Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of  unnatural amino acids in peptides with antimicrobial properties, genetic  incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids.


D-amino acids enzymes natural antibiotics non-natural amino acid synthesis non-proteinogenic amino adics protein synthesis

Editors and affiliations

  • Loredano Pollegioni
    • 1
  • Stefano Servi
    • 2
  1. 1.Dipto. Biotecnologie e di Scienze Mol, Molecolari (DBSM)Università degli Studi dell'InsubriaVareseItaly
  2. 2.Dipto. ChimicaPolitecnico di MilanoMilanoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-330-1
  • Online ISBN 978-1-61779-331-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site