Methods and Protocols

  • Giovanni Manfredi
  • Hibiki Kawamata

Part of the Methods in Molecular Biology book series (MIMB, volume 793)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Neurodegeneration

    1. Front Matter
      Pages 1-1
    2. Hibiki Kawamata, Giovanni Manfredi
      Pages 3-8
    3. Nicole Naniche, Daniela Sau, Piera Pasinelli
      Pages 9-21
    4. Teresa A. Milner, Elizabeth M. Waters, Danielle C. Robinson, Joseph P. Pierce
      Pages 23-59
  3. Neural Cell Culture Techniques

    1. Front Matter
      Pages 61-61
    2. Carol Milligan, David Gifondorwa
      Pages 77-85
    3. Stuart M. Chambers, Yvonne Mica, Lorenz Studer, Mark J. Tomishima
      Pages 87-97
    4. Mark Denham, Mirella Dottori
      Pages 99-110
  4. Models of Neurodegeneration

    1. Front Matter
      Pages 111-111
    2. Alejandro Ocampo, Antoni Barrientos
      Pages 113-127
    3. Michelle L. Tucci, Adam J. Harrington, Guy A. Caldwell, Kim A. Caldwell
      Pages 129-148
    4. Chun-Hung Yeh, Marlon Jansen, Thomas Schmidt-Glenewinkel
      Pages 149-165
    5. Angela S. Laird, Wim Robberecht
      Pages 167-184
    6. Katherine Jackman, Alexander Kunz, Costantino Iadecola
      Pages 195-209
    7. Peter Klivenyi, Laszlo Vecsei
      Pages 211-227
  5. Neurodegenerative Disease Mechanisms: Proteinopathies

    1. Front Matter
      Pages 239-239
    2. Ruben Vidal, Bernardino Ghetti
      Pages 241-258
    3. Han-Xiang Deng, Eileen H. Bigio, Teepu Siddique
      Pages 259-272
    4. Natura Myeku, Maria Jose Metcalfe, Qian Huang, Maria Figueiredo-Pereira
      Pages 273-296
  6. Neurodegenerative Disease Mechanisms: Mitochondria

    1. Front Matter
      Pages 297-297
    2. Christos Chinopoulos, Steven F. Zhang, Bobby Thomas, Vadim Ten, Anatoly A. Starkov
      Pages 311-324
    3. Jianhui Zhu, Ruben K. Dagda, Charleen T. Chu
      Pages 325-339
  7. Neurodegenerative Disease Mechanisms: Biological Functions

    1. Front Matter
      Pages 341-341
    2. Davide Tampellini, Gunnar K. Gouras
      Pages 343-350
    3. Minerva Y. Wong, David Sulzer, Nigel S. Bamford
      Pages 363-376
    4. Carli Lattarulo, Diana Thyssen, Kishore V. Kuchibholta, Bradley T. Hyman, Brian J. Bacskaiq
      Pages 377-389
    5. Yoshie Sugiura, Fujun Chen, Yun Liu, Weichun Lin
      Pages 391-400
  8. Therapeutic Approaches in Neurodegeneration

    1. Front Matter
      Pages 441-441
    2. Peter F. Morgenstern, Roberta Marongiu, Sergei A. Musatov, Michael G. Kaplitt
      Pages 443-455
    3. Chunxing Yang, Linghua Qiu, Zuoshang Xu
      Pages 457-477
    4. Angelo C. Lepore, Nicholas J. Maragakis
      Pages 479-493
    5. Elisabetta Soragni, Chunping Xu, Andrew Cooper, Heather L. Plasterer, James R. Rusche, Joel M. Gottesfeld
      Pages 495-508
  9. Carli Lattarulo, Diana Thyssen, Kishore V. Kuchibholta, Bradley T. Hyman, Brian J. Bacskaiq
    Pages E1-E1
  10. Peter F. Morgenstern, Roberta Marongiu, Sergei A. Musatov, Michael G. Kaplitt
    Pages E3-E3
  11. Back Matter
    Pages 509-512

About this book


Neurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge and present cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Organizes into five sections, chapters detail protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Written in the successful Methods in Molecular Biology™  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neurodegeneration: Methods and Protocols serves as an ideal guide to both novice and professional researchers in the field of neurodegeneration.


Alzheimer's disease Parkinson's disease electron microscopic immunocytochemical methods human neurodegenerative disorders human pluripotent stem cells neurons

Editors and affiliations

  • Giovanni Manfredi
    • 1
  • Hibiki Kawamata
    • 2
  1. 1.Weill Medical College, Dept. Neurology & NeuroscienceCornell UniversityNew YorkUSA
  2. 2.New YorkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-327-1
  • Online ISBN 978-1-61779-328-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site