Methods and Protocols

  • Steven A. Toms
  • Robert J. Weil

Part of the Methods in Molecular Biology book series (MIMB, volume 790)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Preliminary Sample Preparation

    1. Front Matter
      Pages 1-1
    2. Lorenza Franciosi, Natalia Govorukhina, Nick ten Hacken, Dirkje Postma, Rainer Bischoff
      Pages 17-28
  3. Nanoscale Separation

  4. Nanomaterials: Nanostructured Surfaces

    1. Front Matter
      Pages 165-165
    2. Federico Torta, Matteo Fusi, Carlo S. Casari, Andrea Li Bassi, Angela Bachi
      Pages 173-181
    3. Cait Costello, Jan-Ulrich Kreft, Christopher M. Thomas, Paula M. Mendes
      Pages 191-200
  5. Nanomaterials: Nanoscale Tools for Proteomic Discovery

    1. Front Matter
      Pages 201-201
    2. Mohammed Ibrahim Shukoor, Muhammad Nawaz Tahir, Thomas Schladt, Wolfgang Tremel, Zhiqun Zhang, Kevin K. Wang et al.
      Pages 203-214
    3. Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, Serge Muyldermans
      Pages 239-259
  6. Nanotechnology: Techniques in Proteomics – Potential Application for Health and Disease

    1. Front Matter
      Pages 261-261
    2. Michael P. Linnes, Farooq A. Shiekh, Larry W. Hunter, Virginia M. Miller, John C. Lieske
      Pages 263-275
    3. Anton Iliuk, Keerthi Jayasundera, Rachel Schluttenhofer, W. Andy Tao
      Pages 277-285
  7. Back Matter
    Pages 319-322

About this book


As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues.  The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular Biology™, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls.


Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.


Fluidic devices Human pathology Nanomaterials Nanoscale separation Nanotechnologic techniques Nanotechnology Nanotechnology-enhanced detection Proteomics Rare proteins Sample preparation

Editors and affiliations

  • Steven A. Toms
    • 1
  • Robert J. Weil
    • 2
  1. 1., Department of NeurosurgeryGeisinger Health SystemsDanvilleUSA
  2. 2.Neurological Institute, Brain Tumor & Neuro-Oncology CenterThe Cleveland ClinicClevelandUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-318-9
  • Online ISBN 978-1-61779-319-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site