Epigenetics Protocols

  • Trygve O. Tollefsbol

Part of the Methods in Molecular Biology book series (MIMB, volume 791)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Trygve O. Tollefsbol
    Pages 1-10
  3. Yuanyuan Li, Trygve O. Tollefsbol
    Pages 11-21
  4. Ja-Lok Ku, You-Kyung Jeon, Jae-Gahb Park
    Pages 23-32
  5. Thomas Mikeska, Jörg Felsberg, Chelsee A. Hewitt, Alexander Dobrovic
    Pages 33-53
  6. Ida L. M. Candiloro, Thomas Mikeska, Alexander Dobrovic
    Pages 55-71
  7. Cheng-Hong Yang, Yu-Huei Cheng, Li-Yeh Chuang, Hsueh-Wei Chang
    Pages 73-88
  8. Heike Singer, Nicole Nüsgen, Osman El-Maarri
    Pages 89-100
  9. Hisato Okuizumi, Tomoko Takamiya, Yasushi Okazaki, Yoshihide Hayashizaki
    Pages 101-112
  10. Mattia Pelizzola, Annette Molinaro
    Pages 113-123
  11. Natalie Mitchell, J. Tyson DeAngelis, Trygve O. Tollefsbol
    Pages 125-133
  12. Mohsen Karimi, Karin Luttropp, Tomas J. Ekström
    Pages 135-144
  13. Angelica M. Giraldo, Kenneth R. Bondioli
    Pages 145-156
  14. Renata Z. Jurkowska, Alexandre Ceccaldi, Yingying Zhang, Paola B. Arimondo, Albert Jeltsch
    Pages 157-177
  15. Céline Cosseau, Christoph Grunau
    Pages 195-212
  16. Ryan A. Irvine, Cindy Okitsu, Chih-Lin Hsieh
    Pages 213-223
  17. Luke A. Selth, Pierre Close, Jesper Q. Svejstrup
    Pages 253-264
  18. Henriette O’Geen, Lorigail Echipare, Peggy J. Farnham
    Pages 265-286
  19. Takao Nammo, Santiago A. Rodríguez-Seguí, Jorge Ferrer
    Pages 287-296
  20. Yi Huang, Patrick G. Shaw, Nancy E. Davidson
    Pages 297-311
  21. Back Matter
    Pages 329-332

About this book


The field of epigenetics has played a major role at the forefront not only of molecular biology, but also of medical genetics and clinical medicine.  Few disciplines have experienced growth comparable to that which we have witnessed for epigenetics in the past decade. The goal of Epigenetics Protocols, Second Edition is to highlight select techniques that have been mainstays in the field as well as to cover methods that are especially relevant to extant discoveries in epigenetics.  This volume focuses on the two broad areas of epigenetics: DNA methylation and chromatin modifications, and also covers the complex topic of computational methods for epigenetic analyses which is essential to a complete understanding of the vast body of information that is being derived with the use of these newly-developed tools.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Epigenetics Protocols, Second Edition serves as an ideal guide to advanced students, basic scientists and clinical researchers as well as clinicians and biotechnology investigators who wish to continue exploring this exciting and progressive research field.


DNA methylation analysis chromatin immunoprecipitation chromatin modification epigenome polymerase chain reaction methods

Editors and affiliations

  • Trygve O. Tollefsbol
    • 1
  1. 1.Dept. BiologyUniversity of Alabama, BirminghamBirminghamUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-315-8
  • Online ISBN 978-1-61779-316-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site