Methods and Protocols

  • Adalberto Merighi

Part of the Methods in Molecular Biology book series (MIMB, volume 789)

Table of contents

  1. Front Matter
    Pages i-xi
  2. J. Peter H. Burbach
    Pages 1-36
  3. Paolo de Girolamo, Carla Lucini
    Pages 37-56
  4. Marc Landry, Shérine Abdel Salam, Marie Moftah
    Pages 73-87
  5. Harold Gainer, Todd A. Ponzio, Chunmei Yue, Makoto Kawasaki
    Pages 89-110
  6. Laura Lossi, Graziana Gambino, Chiara Salio, Adalberto Merighi
    Pages 111-126
  7. Sarah J. Paulsen, Leif K. Larsen
    Pages 127-135
  8. Joshua J. Park, Y. Peng Loh
    Pages 137-145
  9. Tadahide Furuno, Mamoru Nakanishi
    Pages 171-180
  10. Zhi-Qing David Xu
    Pages 181-189
  11. Fred Nyberg, Mathias Hallberg
    Pages 191-201
  12. Anne-Marie Hesse, Sega Ndiaye, Joelle Vinh
    Pages 203-221
  13. Ping Yin, Xiaowen Hou, Elena V. Romanova, Jonathan V. Sweedler
    Pages 223-236
  14. Mohamed T. Ghorbel, David Murphy
    Pages 237-259
  15. Tetsuya Kushikata, Kazuyoshi Hirota
    Pages 261-269
  16. Rebecca J. Steagall, Carole A. Williams, Arthur W. Duggan
    Pages 271-285
  17. Manuel Ramírez, Isabel Prieto, Inmaculada Banegas, Ana B. Segarra, Francisco Alba
    Pages 287-294
  18. Sergueï O. Fetissov
    Pages 295-302
  19. Michael C. Veronesi, Daniel J. Kubek, Michael J. Kubek
    Pages 303-312
  20. Katalin Prokai-Tatrai, Laszlo Prokai
    Pages 313-336
  21. Frédéric Frézard, Robson A. S. dos Santos, Marco A. P. Fontes
    Pages 343-355
  22. Marijke W. A. de Backer, Keith M. Garner, Mieneke C. M. Luijendijk, Roger A. H. Adan
    Pages 357-376
  23. Isabel Beets, Marleen Lindemans, Tom Janssen, Peter Verleyen
    Pages 377-391
  24. Back Matter
    Pages 393-416

About this book


Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy.  Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field.  The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.


Cellular neurobiology Chemical neuroanatomy Functional analysis Genetic probes Immunocytochemical localization Neurons Protein molecules Therapeutic applications

Editors and affiliations

  • Adalberto Merighi
    • 1
  1. 1., Dipt. di Morfofisiologia VeterinariaUniversità degli Studi di TorinoGrugliascoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-309-7
  • Online ISBN 978-1-61779-310-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site