Protein Microarrays

Methods and Protocols

  • Ulrike Korf

Part of the Methods in Molecular Biology book series (MIMB, volume 785)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Reverse Phase Protein Arrays

    1. Front Matter
      Pages 1-1
    2. Mariaelena Pierobon, Claudio Belluco, Lance A. Liotta, Emanuel F. Petricoin III
      Pages 3-12
    3. Heiko Mannsperger, Stefan Uhlmann, Ulrike Korf, Özgür Sahin
      Pages 45-54
    4. Jan C. Brase, Heiko Mannsperger, Holger Sültmann, Ulrike Korf
      Pages 55-64
    5. Georg Martiny-Baron, Dorothea Haasen, Daniel D’Dorazio, Johannes Voshol, Doriano Fabbro
      Pages 79-107
    6. Daniela Berg, Katharina Malinowsky, Bilge Reischauer, Claudia Wolff, Karl-Friedrich Becker
      Pages 109-122
    7. Claudia Wolff, Christina Schott, Katharina Malinowsky, Daniela Berg, Karl-Friedrich Becker
      Pages 123-140
  3. Antibody Microarrays

    1. Front Matter
      Pages 157-157
    2. Marta Sanchez-Carbayo
      Pages 159-182
    3. Jochen M. Schwenk, Peter Nilsson
      Pages 183-189
    4. Frauke Henjes, Frank Götschel, Anika Jöcker, Ulrike Korf
      Pages 191-201
    5. Christoph Schröder, Mohamed S. S. Alhamdani, Kurt Fellenberg, Andrea Bauer, Anette Jacob, Jörg D. Hoheisel
      Pages 203-221
    6. Johanna Sonntag, Heiko Mannsperger, Anika Jöcker, Ulrike Korf
      Pages 237-245
    7. Carl A. K. Borrebaeck, Christer Wingren
      Pages 247-262
  4. Protein Microarrays

    1. Front Matter
      Pages 263-263
    2. Oda Stoevesandt, Michael J. Taussig, Mingyue He
      Pages 265-276
    3. Holger Erfle, Anastasia Eskova, Jürgen Reymann, Vytaute Starkuviene
      Pages 277-287
    4. David J. O’Connell, Mikael Bauer, Sara Linse, Dolores J. Cahill
      Pages 289-303
    5. Jonathan M. Blackburn, Aubrey Shoko
      Pages 305-330
    6. Sara L. O’Kane, John K. O’Brien, Dolores J. Cahill
      Pages 331-341
  5. Sample Immobilization Strategies

    1. Front Matter
      Pages 343-343
    2. Iain McWilliam, Marisa Chong Kwan, Duncan Hall
      Pages 345-361
    3. John Austin, Antonia H. Holway
      Pages 379-394
  6. Back Matter
    Pages 395-398

About this book


Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of hundreds of proteins in thousands of samples is attainable.  In Protein Microarrays: Methods and Protocols, expert researchers in the field present an up-to-date collection of robust strategies in the field of protein microarrays and summarize recent advantages in the field of printing technologies, the development of suitable surface materials, as well as detection and quantification technologies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key notes on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, Protein Microarrays: Methods and Protocols aims to stimulate the application and further advancement of this powerful technology in labs worldwide.


Antibodies Cellular proteome Functional proteomics Immobilization Printing technologies Protein function Reverse phase Signaling network regulation Systems biology

Editors and affiliations

  • Ulrike Korf
    • 1
  1. 1.(DKFZ), Division of Molecular Genome AnalysisDeutsches KrebsforschungszentrumHeidelbergGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-285-4
  • Online ISBN 978-1-61779-286-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site