Single Molecule Analysis

Methods and Protocols

  • Erwin J. G. Peterman
  • Gijs J. L. Wuite

Part of the Methods in Molecular Biology book series (MIMB, volume 783)

Table of contents

  1. Front Matter
    Pages i-x
  2. Joost van Mameren, Gijs J. L. Wuite, Iddo Heller
    Pages 1-20
  3. Katherine H. White, Koen Visscher
    Pages 21-43
  4. Ismaïl Cissé, Pierre Mangeol, Ulrich Bockelmann
    Pages 45-61
  5. Arne Gennerich, Samara L. Reck-Peterson
    Pages 63-80
  6. Siet M. J. L. van den Wildenberg, Bram Prevo, Erwin J. G. Peterman
    Pages 81-99
  7. Mauro Modesti
    Pages 101-120
  8. Till Korten, Bert Nitzsche, Chris Gell, Felix Ruhnow, Cécile Leduc, Stefan Diez
    Pages 121-137
  9. Fredrik Persson, Fredrik Westerlund, Jonas O. Tegenfeldt
    Pages 159-179
  10. Patrick Ferrand, Jérôme Wenger, Hervé Rigneault
    Pages 181-195
  11. Pedro J. de Pablo
    Pages 197-212
  12. Dejan Ristic, Humberto Sanchez, Claire Wyman
    Pages 213-231
  13. Yeonee Seol, Keir C. Neuman
    Pages 265-293
  14. David Dunlap, Chiara Zurla, Carlo Manzo, Laura Finzi
    Pages 295-313
  15. Back Matter
    Pages 315-317

About this book


Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.


atomic force microscopy magnetic tweezers optical tweezers single molecule fluorescence methods tethered particle motion

Editors and affiliations

  • Erwin J. G. Peterman
    • 1
  • Gijs J. L. Wuite
    • 2
  1. 1.Dept. Physics & Astronomy, Section Physics of Complex SystemsVrije Universiteit AmsterdamAmsterdamNetherlands
  2. 2.Dept. Physics & Astronomy, Section Physics of Complex SystemsVrije Universiteit AmsterdamAmsterdamNetherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-281-6
  • Online ISBN 978-1-61779-282-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site