Poly(ADP-ribose) Polymerase

Methods and Protocols

  • Alexei V. Tulin

Part of the Methods in Molecular Biology book series (MIMB, volume 780)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Biochemical Approaches, In Vitro and In Vivo

    1. Front Matter
      Pages 1-1
    2. Girish M. Shah, Febitha Kandan-Kulangara, Alicia Montoni, Rashmi G. Shah, Julie Brind’Amour, Momchild́ D. Vodenicharov et al.
      Pages 3-34
    3. Opeyemi A. Olabisi, Chi-Wing Chow
      Pages 47-55
    4. Florian Rosenthal, Simon Messner, Bernd Roschitzki, Peter Gehrig, Paolo Nanni, Michael O. Hottiger
      Pages 57-66
    5. Maria Malanga, Felix R. Althaus
      Pages 67-82
    6. Jean-Philippe Gagné, Jean-François Haince, Émilie Pic, Guy G. Poirier
      Pages 93-115
    7. Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, Robert S. B. Clark
      Pages 117-134
    8. Jean-Christophe Amé, Thomas Kalisch, Françoise Dantzer, Valérie Schreiber
      Pages 135-152
    9. Cynthia M. Simbulan-Rosenthal, Dean S. Rosenthal, Mark E. Smulson
      Pages 165-190
    10. Marie-France Langelier, Jamie L. Planck, Kristin M. Servent, John M. Pascal
      Pages 209-226
    11. Mi Young Kim
      Pages 227-236
  3. Genetic Manipulation Approaches

    1. Front Matter
      Pages 237-237
    2. Dawn Farrar, Igor Chernukhin, Elena Klenova
      Pages 293-312
    3. Christian Boehler, Laurent Gauthier, Jose Yelamos, Aurélia Noll, Valérie Schreiber, Françoise Dantzer
      Pages 313-336
    4. Mirella L. Meyer-Ficca, Ralph G. Meyer
      Pages 349-376
    5. Hiroto Nodono, Akiko Hamada, Yasuhito Kuroda, Kazuo Kamemura, Makoto Hasegawa, Toru Komiya et al.
      Pages 377-390
    6. Jean-François St-Laurent, Serge Desnoyers
      Pages 413-425
    7. Anna De Maio, Elena Porzio, Ida Romano, Barbara Nicolaus, Maria Rosaria Faraone Mennella
      Pages 443-460
  4. Discovery of New PARP Inhibitors

    1. Front Matter
      Pages 461-461
    2. Stacie Canan, Karen Maegley, Nicola Curtin
      Pages 463-489
    3. Elena Kotova, Aaron D. Pinnola, Alexei V. Tulin
      Pages 491-516
    4. Anna De Maio, Elena Porzio, Ida Romano, Barbara Nicolaus, Maria Rosaria Faraone Mennella
      Pages E1-E1
  5. Back Matter
    Pages 517-521

About this book


Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous proteins that regulate crucial processes of the cell cycle, DNA repair, genomic stability, and transcriptional regulation.  Being involved in basic cell functions, PARPs mediate rapid responses to such environmental factors as stress, infection, nutrition and hormonal signals. Whereas PARP inhibitors can suppress tumor growth and proliferation in certain breast, ovarian, and prostate cancers, understanding how PARP controls cellular functions is essential for the development of novel cancer treatments strategies. Divided into three convenient sections, Poly(ADP-Ribose) Polymerase: Methods and Protocols aims to explain how PARP proteins act within the normal development of an organism as well as in pathogenic conditions, seeks to advance the knowledge of developmental pathways regulation, and endeavors to facilitate the development of new therapeutic drugs and methods to target PARP-dependent processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Poly(ADP-Ribose) Polymerase: Methods and Protocols serves as an ideal guide to scientists who wish to continue exploring this exciting and progressive research field.



PARP inhibitors PARP1 genomics drosophila mammalian genome mass spectrometry non-covalent pADPr interactions

Editors and affiliations

  • Alexei V. Tulin
    • 1
  1. 1.Keystone Program in Epigenetics &, Progenitor CellsFox Chase Cancer CenterPhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-269-4
  • Online ISBN 978-1-61779-270-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site