Book Volume 779 2011

Plant Kinases

Methods and Protocols

Editors:

ISBN: 978-1-61779-263-2 (Print) 978-1-61779-264-9 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-x

  2. Overview

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-5

      Guide to the Book Plant Kinases

    3. No Access

      Protocol

      Pages 7-52

      The Age of Protein Kinases

  3. Kinase Expression, Purification and Activity Assays

    1. Front Matter

      Pages 53-53

    2. No Access

      Protocol

      Pages 55-63

      Expression and Purification of Active Protein Kinases from Wheat Germ Extracts

    3. No Access

      Protocol

      Pages 65-78

      Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System

    4. No Access

      Protocol

      Pages 79-92

      Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants

    5. No Access

      Protocol

      Pages 93-138

      Use of Phospho-Site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks

    6. No Access

      Protocol

      Pages 139-147

      Bacterial Assay to Study Plant Sensor Histidine Kinases

    7. No Access

      Protocol

      Pages 149-161

      Substrate Analysis of Arabidopsis PP2C-Type Protein Phosphatases

  4. Studying Kinase Function at the Cellular Level

    1. Front Matter

      Pages 163-163

    2. No Access

      Protocol

      Pages 165-183

      Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen

    3. No Access

      Protocol

      Pages 185-197

      Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules

    4. No Access

      Protocol

      Pages 199-215

      Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization

    5. No Access

      Protocol

      Pages 217-224

      Quantifying Degradation Rates of Transmembrane Receptor Kinases

    6. No Access

      Protocol

      Pages 225-242

      Fluorescence Correlation Spectroscopy and Fluorescence Recovery After Photobleaching to Study Receptor Kinase Mobility In Planta

  5. Identification of Kinase Substrates and Phosphorylation-Site Detection

    1. Front Matter

      Pages 243-243

    2. No Access

      Protocol

      Pages 245-257

      Bimolecular-Fluorescence Complementation Assay to Monitor Kinase–Substrate Interactions In Vivo

    3. No Access

      Protocol

      Pages 259-271

      Chemical Genetic Analysis of Protein Kinase Function in Plants

    4. No Access

      Protocol

      Pages 273-286

      Modified Metal-Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes

    5. No Access

      Protocol

      Pages 287-302

      Phosphoproteomics Using iTRAQ

  6. Back Matter

    Pages 303-312