Plant Kinases

Methods and Protocols

  • Nico Dissmeyer
  • Arp Schnittger

Part of the Methods in Molecular Biology book series (MIMB, volume 779)

Table of contents

  1. Front Matter
    Pages i-x
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Nico Dissmeyer, Arp Schnittger
      Pages 3-5
    3. Nico Dissmeyer, Arp Schnittger
      Pages 7-52
  3. Kinase Expression, Purification and Activity Assays

  4. Studying Kinase Function at the Cellular Level

    1. Front Matter
      Pages 163-163
    2. Shutian Li, Vernonica E. Franklin-Tong
      Pages 165-183
    3. Dor Salomon, Chao Zhang, Kevan M. Shokat, Guido Sessa
      Pages 185-197
    4. Mark A. Hink, Sacco C. de Vries, Antonie J. W. G. Visser
      Pages 199-215
    5. Mark Kwaaitaal, Marieke Schor, Mark A. Hink, Antonie J. W. G. Visser, Sacco C. de Vries
      Pages 225-242
  5. Identification of Kinase Substrates and Phosphorylation-Site Detection

    1. Front Matter
      Pages 243-243
    2. Maik Böhmer, Michael Bölker, Tina Romeis
      Pages 259-271

About this book

Introduction

Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to  both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.

Keywords

active protein kinase kinase substrate phosphoproteomes phosphorylation-site detection pollen apoptosis

Editors and affiliations

  • Nico Dissmeyer
    • 1
  • Arp Schnittger
    • 2
  1. 1.Leibniz Institute of Plant BiochemistryHalle (Saale)Germany
  2. 2.StrasbourgFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-264-9
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-263-2
  • Online ISBN 978-1-61779-264-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book