Chloroplast Research in Arabidopsis

Methods and Protocols, Volume I

  • R. Paul Jarvis

Part of the Methods in Molecular Biology book series (MIMB, volume 774)

Table of contents

  1. Front Matter
    Pages i-x
  2. Genetics, Cytology, and In Vivo Analysis

  3. Gene Expression and Protein Accumulation

    1. Front Matter
      Pages 149-149
    2. Beth A. Rowan, Arnold J. Bendich
      Pages 151-170
    3. Yan O. Zubo, Thomas Börner, Karsten Liere
      Pages 171-182
    4. Lars L. E. Sjögren, Adrian K. Clarke
      Pages 225-240
  4. Protein Transport, Localization, and Topology

  5. Back Matter
    Pages 369-374

About this book


Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume I explores topics such as genetics, cytology, in vivo analysis, gene expression, and protein accumulation, as well as protein transport, localization, and topology.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.


Arabidopsis thaliana Cytology Gene expression Model system Photosynthesis Plant genetics Plastid research Protein accumulation Protein localization Protein transport and topology

Editors and affiliations

  • R. Paul Jarvis
    • 1
  1. 1., Department of BiologyUniversity of LeicesterLeicesterUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-233-5
  • Online ISBN 978-1-61779-234-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site